MIDAS workflow000000 (MRM-triggered MS/MS) verification of the identity of peptide DLQFVEVTDVK representing fibronectin (normal concentration, ∼300 μg/ml)

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MIDAS workflow000000 (MRM-triggered MS/MS) verification of the identity of peptide DLQFVEVTDVK representing fibronectin (normal concentration, ∼300 μg/ml) in a digest of depleted human plasma.A shows the ion current profile of MRM 647.3/789.4 (MS1/MS2), and B shows the MS/MS spectrum of peptide fragments taken at the time of the peak (* marks the y7 fragment monitored in this MRM). cps, counts per second. MIDAS workflow000000 (MRM-triggered MS/MS) verification of the identity of peptide DLQFVEVTDVK representing fibronectin (normal concentration, ∼300 μg/ml) in a digest of depleted human plasma.A shows the ion current profile of MRM 647.3/789.4 (MS1/MS2), and B shows the MS/MS spectrum of peptide fragments taken at the time of the peak (* marks the y7 fragment monitored in this MRM). cps, counts per second. Leigh Anderson, and Christie L. Hunter Mol Cell Proteomics 2006;5:573-588 © 2006 The American Society for Biochemistry and Molecular Biology