S. A. Gomez, F. G. Pasteran, D. Faccone, N. Tijet, M. Rapoport, C

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Clonal dissemination of Klebsiella pneumoniae ST258 harbouring KPC-2 in Argentina S.A. Gomez, F.G. Pasteran, D. Faccone, N. Tijet, M. Rapoport, C. Lucero, O. Lastovetska, E. Albornoz, M. Galas, R.G. Melano, A. Corso, A. Petroni Clinical Microbiology and Infection Volume 17, Issue 10, Pages 1520-1524 (October 2011) DOI: 10.1111/j.1469-0691.2011.03600.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Species distribution of the 77 blaKPC-2-producing isolates in the different time periods. The first two KPC-producing isolates collected in 2006 (Citrobacter freundii and Klebsiella pneumoniae NON-ST258) were excluded. For each time period, the number of hospitals where blaKPC-2-producing isolates were recovered, and the numbers of Kpn-ST258, Kpn-NON-ST258 and other enterobacteria, are shown. Kpn, K. pneumoniae. Clinical Microbiology and Infection 2011 17, 1520-1524DOI: (10.1111/j.1469-0691.2011.03600.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Genetic environment of blaKPC-2 in enterobacteria from Argentina. Genes (open reading frames) and their corresponding transcriptional orientations are indicated by horizontal broad arrows. Filled and empty triangles represent inverted repeats of insertion sequences (ISs) or Tn3-group transposons, respectively. (a) The Tn4401a sequence was from plasmid pNYC (Gen-Bank accession number EU176011) and the region of the plasmid pKP048 depicted corresponds to coordinates 15 736 to 25 596 (complement) in Gen-Bank FJ628167. Variant 1a represents the sequence obtained from Citrobacter freundii M9169 and Enterobacter cloacae M11180 [9] (10 047 bp, Gen-Bank JN048639 and JN048640, respectively). ΔblaTEM-1 indicates a deletion of 10 bp upstream of the blaTEM-1 start plus the first 291 nucleotides of the encoding gene for the Tn3 sequence. Only the 58-bp deleted fragment in Variant 1b is shown, indicating the Tn801-like inverted repeat right (underlined text) and its A/T-rich pentameric target site duplication (TSD). The grey-shaded areas show identical regions among the compared structures. Thick lines with single or double arrowheads indicate the different ISs or transposons found, and thin lines with double arrowheads indicate the identity with plasmid pFBAOT6 from Aeromonas punctata (Gen-Bank CR376602). (b) PCR mapping used for characterizing Tn4401a-harbouring (upper) or Variant 1a/b-harbouring isolates (lower). The amplicons obtained in the different PCR assays and the lengths (bp) of the different amplicons are represented by thick lines. The letters and numbers at the left of each line indicate the different PCR used (primers are listed in the Supporting Information). Amplicons 7 and 8 were sequenced in all isolates to discriminate between Variants 1a and 1b. Clinical Microbiology and Infection 2011 17, 1520-1524DOI: (10.1111/j.1469-0691.2011.03600.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions