Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1- CreERT2 transgenic mice M. Zhu, M.D., Ph.D., M. Chen, Ph.D., A.C. Lichtler,

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Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1- CreERT2 transgenic mice M. Zhu, M.D., Ph.D., M. Chen, Ph.D., A.C. Lichtler, Ph.D., R.J. O'Keefe, M.D., Ph.D., D. Chen, M.D., Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 1, Pages 129-130 (January 2008) DOI: 10.1016/j.joca.2007.08.001 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Histological analyses of the Col2a1-Cre+/−ERT2;R26R+/− mice. Two-week-old Col2a1-Cre+/−ERT2;R26R+/− double transgenic mice and Cre-negative control littermates were injected with TM (1mgTM/mouse/day for 5 days). Mice were sacrificed 1 (a) and 6 months (b) after injections were completed and samples of long bones were fixed, decalcified and processed for frozen section preparation followed by X-Gal staining. Sections were then counterstained with Nuclear Fast Red. Articular chondrocytes of double transgenic mice that received TM showed X-Gal-positive staining (a,b). Recombination efficiency was evaluated by counting the X-Gal-positive cells divided by total cell numbers in articular cartilage area from three non-consecutive histological sections of four transgenic mice (n=4). High Cre-recombination efficiency was achieved in articular chondrocytes of the transgenic mice 1 and 6 months after TM induction. Osteoarthritis and Cartilage 2008 16, 129-130DOI: (10.1016/j.joca.2007.08.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions