Evaluation of alternative cell lines for the isolation of FMDV

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Evaluation of alternative cell lines for the isolation of FMDV Ashley Gray, Valerie Mioulet, Britta Wood, Elizabeth Reid, Lissie Henry, Barsha Thapa, Donald King

Scope Virus isolation remains key component in FMDV diagnostics Cells currently used in the WRL: Primary bovine thyroid: expensive, labour intensive, require experienced staff IB-RS-2 pig kidney: Persistently infected with CSF and therefore, requirement for biosafety provisions Purpose: Investigate the FMDV sensitivity of other continuous cell lines for use in diagnostics

Sensitivity comparisons Figure 2: The LFBK-αVβ6 cell line produced consistently higher viral titres (logTCID50) (average + SD) than IB-RS-2 and ZZ-R for isolate HKN 1/2015 type O (CATHAY topotype). The number of titrations conducted to-date are shown on each bar (n). n=2 n=3 n=4

Future work Sensitivity comparisons using clinical samples Validation required for ISO/IEC 17025 accreditation Development of a continuous BTY cell line expressing high levels of αVβ6 integrin receptor Acknowledgement With thanks to L. Rodrigues and M. LaRocco, Plum Island, USDA/ARS, for providing the LFBK- αVβ6 cell line.