Studies on wheat straw and wood chips degradation in soil By Mythreyi Chandoor.

Contents Work accomplished till now Results and Discussion New Ideas

The basic idea was to incubate the wheat straw and wood chips Objective : Main Objective: The basic idea was to incubate the wheat straw and wood chips for incubation in Soil to look at its rate of degradation and model the system and also to find the soil Microcosm responsible for Lignocellulosic Degradation.

Work accomplished till now Soil Bags filled with wheat straw and wood chips Pint Jar Type : Palouse Moisture : 20% Carbon : 2.285 Sulphur: 0.02697 Phosphorous: 0.1711 Oven dried samples of wheat straw and wood chips Autoclaved pint jars Jars = 40 ,a set of 4 for every 4 weeks incubation temperature = 21 0 centigrade Control = soil .

Serial Dilutions of Soil Elementary analysis Serial Dilutions of Soil Media used : LB and PDA Wheat straw samples Media used : LB and PDA Wood samples Media used : LB AND PDA

PDA + Azure and LBA +Azure Selective media ,growth on Azo-Dyes Control PDA + Azure and LBA +Azure Overnight incubation At 30 degrees centigrade

Discolouration in Broth media (poly dyes and Azodyes ) sno Absorbance at 370nm for Poly -R 10-5 0.264 0.257 10-2 0.258 10-1 0.261 STRAW 0.579 0.586 10-6 0.253 10-4 0.270 Poly –R Dye Dilutions Absorbance At 370 nm 100 0.572 10 0.063 1 0.014 0.5 0.004 0.1 0.003 sno Absorbance at 370nm for Poly -B 10-5 0.585 0.581 10-2 0.273 0.276 10-1 0.664 0.663 STRAW 0.257 0.264 10-6 0.245 0.243 10-4 0.248 Poly –B Dye Dilutions Absorbance At 370 nm 100 0.534 0.281 10 0.181 1 0.024 0.5 0.035 0.1 0.001 sno Absorbance at 370nm for LB media 10-5 0.369 0.356 10-2 0.390 0.475 10-1 0.384 0.389 STRAW 0.352 10-6 0.364 0.377 10-4 0.351 0.370 LB +azure Dilutions Absorbance At 370 nm 100 0.783 10 0.080 1 0.015 0.5 0.001 0.1 0.000 50 0.343 50 0.281 50 0.407

Elementary analysis of Wheat Straw (GC –MS Pyrolysis) CO2 Elementary analysis of Wheat Straw (GC –MS Pyrolysis)

Elementary analysis of Wood Chips (GC –MS Pyrolysis)

Pretreatment and Fermentation New idea !! Feedstock Cellulose Hemicellulose Lignin Ratio Pretreatment and Fermentation Input : Feedstock Database1 Feedstock Database 2 Microcosm/ Thermo chemical combinations. Database3 Cost efficiency Output The Process cost structure products