Promega Corporation: Technology Areas of Interest Heather Gerard Intellectual Property Manager

Our Mission Provide innovative biological reagents and integrated systems used in research and applied technology worldwide.

Highlights Founded in 1978 Headquartered in Madison, Wisconsin Calendar Year 2016 Revenues Approaching 400M USD ~1,400 employees in 16 countries Over 3,500 products for life science research and applied science distributed in >100 countries Proprietary Information. Not for further distribution.

Commercial and Manufacturing Sites Proprietary Information. Not for further distribution.

Sales by Region of the World Proprietary Information. Not for further distribution.

Breadth of Support Proprietary Information. Not for further distribution.

Sales by Primary Market

Breadth of Support

Innovation Through Research and Development

Cellular and Biochemical Technologies Applied and Environmental Analysis Assay Design (including custom design) Integrated Cellular Biology Macromolecular Design Organic Chemistry Protein Analysis Proprietary Information. Not for further distribution.

Nucleic Acid Technologies Amplification Applied and Environmental Analysis Detection Human Genetic Identity Purification Proprietary Information. Not for further distribution.

Integrated Automation Instrumentation Reagents Software Services Proprietary Information. Not for further distribution.

Capabilities Advanced Technology Group Custom Assay Service Custom Application Development Automated Protocol Development Customized Product Development for OEM Manufacture In and Out Licensing Proprietary Information. Not for further distribution.

Areas of Interest

Genetic Analysis: Areas of Interest Novel, thermostable or high fidelity DNA polymerases Faster enzymes for integrated devices Gene Methylation and Epigenetics Automated RNA purification (for use on Maxwell) Phenotypic and genotypic markers for human identity testing RNA and genomic DNA purification technologies Micro RNA purification and analysis Nucleic acid stabilization Differential tissue/cell extraction from samples Novel ways of high throughput DNA concentration

Cellular Analysis: Areas of Interest Cell-based assays for discovery and development of drug leads, including assays of efficacy, specificity, and safety Bioassay technologies for development, evaluation, and production of biopharmaceuticals Technologies for manipulation, evaluation and analysis of stem cells, progenitor cells, and differentiated cells Novel transfection/infection methods for gene delivery into mammalian cells, especially stem cells Lentivirus vectors

Proteomics: Areas of Interest Methods for preparing and analyzing samples by mass spec, especially for quantitative analysis of sample composition and protein structure Methods for detection of post-translational modifications (e.g., phosphorylation, ubiquitinylation, acetylation, glycosylation, etc.) and enzymes involved in their creation Methods for analyzing protein function in cells, cell lysates, and immobilized on surfaces (e.g., interactions, enzyme activities, etc.) Methods for development and evaluation of biopharmaceuticals (e.g., antibodies or recombinant protein therapeutics) Eukaryotic systems for in vitro synthesis of large amounts of active, soluble proteins New functionalities for HaloTag, including novel surfaces for protein capture, analysis, and purification

Bioluminescent Technologies : (including optical imaging) Areas of Interest Bioluminescent Technologies : (including optical imaging) Novel bioluminescent chemistries (e.g. luciferases, auxiliary enzymes, etc.) Novel mutant luciferases Novel bioluminescent substrate derivatives Novel assay designs Luciferins/pro-luciferins

Chemistry: Areas of Interest Red ligands: >600nm emission, as bright as xanthene dyes in water Dyes that sense environmental changes (pH, ionic concentration, etc.) Novel intracellular bioluminescent or fluorescent probes Novel luminophores for cellular analysis Novel fluorescent dyes for labeling and detecting nucleic acid Chemically cleavable linkers Solid phase formulation additive for stability and solubility Protein labeling-high affinity and specific; intracellular and extracellular Activity based protein profiling Photoaffinity labeling reagents Turn on fluorogenic dyes Novel click and bio-orthogonal chemistry

Next generation sequencing: Areas of Interest Next generation sequencing: Single cell analysis Technologies to enhance sequencing instrument use PCR cleanup and DNA sizing Direct library prep from samples for e.g. FFPE Epigenetic analysis Niche applications

Instrumentation: Areas of Interest Automated Nucleic Acid Purification Additional capabilities for Maxwell system Sample pre-processing Plasmid purification Integrated Solutions Maxwell application expansion Instrumentation related to Promega’s reagents Field-compatible instrumentation for genetic analysis (DNA purification, rapid amplification, separation and analysis) qPCR instruments Liquid handling instruments Automated punch instruments for sample processing

Areas of Little Interest Specific therapeutic agents or genes/proteins Manual plasmid DNA purification Site-directed mutagenesis DNA sequencing siRNA/RNAi Standard transfection technologies Antibodies Prokaryotic expression vectors ESC or iPS cell lines or differentiated cell lines derived from them. (Promega will cooperate with entities producing such cell lines to enable them to use Promega’s reporter genes, HaloTag, or other technologies in their cell lines including cooperation in licensing) Label-free technologies Ab based clinical diagnostics Infectious disease clinical diagnostics

Technology Acquisition Team Preeta Guptan Technology Acquisition Scout preeta.guptan@promega.com (608) 443-3208 Tim McCauley Licensing Manager tim.mccauley@promega.com (608) 277-2577 Dr. Randall L. Dimond VP, Chief Technical Officer randy.dimond@promega.com (608) 277-2517 23

Promega Licensing Portal 24

Questions Welcome