Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage Y. Yu, H. Zheng, J.A. Buckwalter, J.A. Martin

Slides:

Advertisements

Similar presentations

M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M

Advertisements

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.

Expression pattern differences between osteoarthritic chondrocytes and mesenchymal stem cells during chondrogenic differentiation P. Bernstein, C. Sticht,

Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K.

Chondrocytes extract from patients with osteoarthritis induces chondrogenesis in infrapatellar fat pad-derived stem cells E. López-Ruiz, M. Perán, J.

Time-lapse observation of the dedifferentiation process in mouse chondrocytes using chondrocyte-specific reporters Y. Minegishi, K. Hosokawa, N. Tsumaki

Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation Y. Park, J. Hosomichi,

Muscle cell-derived factors inhibit inflammatory stimuli-induced damage in hMSC- derived chondrocytes R.S. Rainbow, H. Kwon, A.T. Foote, R.C. Preda, D.L.

Moderate dynamic compression inhibits pro-catabolic response of cartilage to mechanical injury, tumor necrosis factor-α and interleukin-6, but accentuates.

Glycoprotein Nonmelanoma Clone B Regulates the Crosstalk between Macrophages and Mesenchymal Stem Cells toward Wound Repair Bing Yu, Talib Alboslemy,

Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal.

Regulation of mesenchymal stem cell chondrogenesis by glucose through protein kinase C/transforming growth factor signaling T.-L. Tsai, P.A. Manner,

Granulocyte macrophage – colony stimulating factor (GM-CSF) significantly enhances articular cartilage repair potential by microfracture M.-D. Truong,

Stepwise preconditioning enhances mesenchymal stem cell-based cartilage regeneration through epigenetic modification S. Lin, W.Y.W. Lee, L. Xu, Y. Wang,

Volume 7, Issue 2, Pages (August 2016)

M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M

Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K.

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.

X. Zhang, I. Prasadam, W. Fang, R. Crawford, Y. Xiao

Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes Li-Bo Jiang,

Deiodinase 2 upregulation demonstrated in osteoarthritis patients cartilage causes cartilage destruction in tissue-specific transgenic rats H. Nagase,

Human articular chondrocytes with higher aldehyde dehydrogenase activity have stronger expression of COL2A1 and SOX9 A. Unguryte, E. Bernotiene, E. Bagdonas,

Subtractive gene expression profiling of articular cartilage and mesenchymal stem cells: serpins as cartilage-relevant differentiation markers S. Boeuf,

Osteochondral defect repair using bilayered hydrogels encapsulating both chondrogenically and osteogenically pre-differentiated mesenchymal stem cells.

Mechanical loading regimes affect the anabolic and catabolic activities by chondrocytes encapsulated in PEG hydrogels G.D. Nicodemus, S.J. Bryant Osteoarthritis.

Functional consequences of glucose and oxygen deprivation on engineered mesenchymal stem cell-based cartilage constructs M.J. Farrell, J.I. Shin, L.J.

The role of the PCM in reducing oxidative stress induced by radical initiated photoencapsulation of chondrocytes in poly(ethylene glycol) hydrogels N.

J.E. Lafont, F.-A. Poujade, M. Pasdeloup, P. Neyret, F. Mallein-Gerin

Endoplasmic reticulum stress-induced apoptosis contributes to articular cartilage degeneration via C/EBP homologous protein Y. Uehara, J. Hirose, S.

Inhibition of Notch Signaling Promotes the Adipogenic Differentiation of Mesenchymal Stem Cells Through Autophagy Activation and PTEN-PI3K/AKT/mTOR Pathway.

Interaction between zonal populations of articular chondrocytes suppresses chondrocyte mineralization and this process is mediated by PTHrP J. Jiang,

G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage

MicroRNA-320 regulates matrix metalloproteinase-13 expression in chondrogenesis and interleukin-1β-induced chondrocyte responses F. Meng, Z. Zhang, W.

Functional cartilage repair capacity of de-differentiated, chondrocyte- and mesenchymal stem cell-laden hydrogels in vitro L. Rackwitz, F. Djouad, S.

Depletion of primary cilia in articular chondrocytes results in reduced Gli3 repressor to activator ratio, increased Hedgehog signaling, and symptoms.

Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture Dr R.L. Mauck, Ph.D., X. Yuan, Dr.

CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFβ and BMP through SMAD signaling B. Saitta,

Toward scaffold-based meniscus repair: effect of human serum, hyaluronic acid and TGF-ß3 on cell recruitment and re-differentiation U. Freymann, M. Endres,

The impact of forced joint exercise on lubricin biosynthesis from articular cartilage following ACL transection and intra-articular lubricin's effect.

H.H. Lee, M.J. O'Malley, N.A. Friel, C.R. Chu

Osmolarity effects on bovine articular chondrocytes during three-dimensional culture in alginate beads X. Xu, J.P.G. Urban, U.K. Tirlapur, Z. Cui Osteoarthritis.

Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: implications for bone repair during inflammation D.C. Lacey, P.J. Simmons,

Human synovial fluid derived mesenchymal stem cells expanded under low oxygen conditions and in a serum-free environment exhibit enhanced lineage-specific.

Enrichment of committed human nucleus pulposus cells expressing chondroitin sulfate proteoglycans under alginate encapsulation Y. Sun, M. Lv, L. Zhou,

Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that.

M.M.-G. Sun, F. Beier Osteoarthritis and Cartilage

Involvement of Gas7 along the ERK1/2 MAP kinase and SOX9 pathway in chondrogenesis of human marrow-derived mesenchymal stem cells Y. Chang, M.D., S.W.N.

Perturbations in the HDL metabolic pathway predispose to the development of osteoarthritis in mice following long-term exposure to western-type diet

Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins M. Pei, M.D., Ph.D., J. Luo, M.D.,

M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M

Osteochondral defect repair using bilayered hydrogels encapsulating both chondrogenically and osteogenically pre-differentiated mesenchymal stem cells.

T. Kurth, M. Sc. , E. Hedbom, Ph. D. , N. Shintani, Ph. D. , M

L. C. Davies, B. Sc. , Ph. D. , E. J. Blain, B. Sc. , Ph. D. , B

Pharmaceutical nanocarrier association with chondrocytes and cartilage explants: influence of surface modification and extracellular matrix depletion

Growth characterization of neo porcine cartilage pellets and their use in an interactive culture model Carsten Lübke, Ph.D., Jochen Ringe, M.Sc., Veit.

Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins M. Shimaya, T. Muneta, S. Ichinose, K. Tsuji,

Cartilaginous repair of full-thickness articular cartilage defects is induced by the intermittent activation of PTH/PTHrP signaling S. Kudo, H. Mizuta,

J. A. Martin, A. Martini, A. Molinari, W. Morgan, W. Ramalingam, J. A

L. De Franceschi, Ph. D. , L. Roseti, Ph. D. , G. Desando, Ph. D. , A

Volume 4, Issue 3, Pages (March 2015)

C. Zhou, H. Zheng, J.A. Martin Osteoarthritis and Cartilage

PTHrP overexpression partially inhibits a mechanical strain-induced arthritic phenotype in chondrocytes D. Wang, J.M. Taboas, R.S. Tuan Osteoarthritis.

Kim L. Kroeze, Wouter J. Jurgens, Behrouz Z. Doulabi, Florine J

Estrogen reduces mechanical injury-related cell death and proteoglycan degradation in mature articular cartilage independent of the presence of the superficial.

Optimal ratio of adipose stem cells and bone marrow stem cell to promote osteogenic differentiation and angiogenesis G.-I. Im, K.-I. Kim, N.-H. Jo Osteoarthritis.

Enhanced phagocytic capacity endows chondrogenic progenitor cells with a novel scavenger function within injured cartilage C. Zhou, H. Zheng, J.A. Buckwalter,

Demineralized bone alters expression of Wnt network components during chondroinduction of post-natal fibroblasts Karen E. Yates, Ph.D Osteoarthritis.

Volume 8, Issue 6, Pages (June 2017)

Volume 22, Issue 11, Pages (November 2014)

Structured three-dimensional co-culture of mesenchymal stem cells with chondrocytes promotes chondrogenic differentiation without hypertrophy M.E. Cooke,

Presentation transcript:

Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage Y. Yu, H. Zheng, J.A. Buckwalter, J.A. Martin Osteoarthritis and Cartilage Volume 22, Issue 9, Pages 1318-1326 (September 2014) DOI: 10.1016/j.joca.2014.07.002 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Experimental schematics and customized apparatus. A) Flow diagram representing the methodology for isolating and characterizing cartilage progenitor cells. B) A customize-made apparatus for separating superficial and deep cartilage from 6 mm cartilage biopsy, with a caliper, and sample holder, and a blade socket. C) Histological image showing full thickness bovine articular cartilage (Scale bar represents 500 μm). Osteoarthritis and Cartilage 2014 22, 1318-1326DOI: (10.1016/j.joca.2014.07.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Fluorescent images of different types of colonies and their relative ratio and dimension. A) Small colony. B) Big colony. C) Confluent colony. D) The percentage of different type of colonies from full thickness cartilage, and superficial 1/3 and deep 2/3, respectively. E) The criteria used for categorized colonies in regard to their size and cell number. (Scale bars = 200 microns) F) Non-colony forming cells with characteristic cobblestone-like shape of chondrocytes. G) CFCs displayed more stretched fibroblast-like morphology similar to mesenchymal stem cells. (Scale bar = 50 microns). Osteoarthritis and Cartilage 2014 22, 1318-1326DOI: (10.1016/j.joca.2014.07.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Gene expression of HCCs (BCs and CCs). A, B) Real-time qRT-PCR and/or agarose gel electrophoresis showed significant overexpression of stem/progenitor cell makers ABCG2, TERT and Notch1 in BCs and CCs compared with NCs. C) A array of MSCs markers were significantly over-expressed level in BCs and CCs vs NCs. D) Higher expression of chondrogenic transcription factor SOX-9 and osteogenic transcription factor RUNX-2 in BCs and CCs relative to NCs. E) CCs showed positive staining for both ABCG2 (upper right) and Notch-1 (lower right) in comparison to NCs. (Scale bar represents 50 μm). The error bars in each plot represent the 95% confidence interval (CI). Osteoarthritis and Cartilage 2014 22, 1318-1326DOI: (10.1016/j.joca.2014.07.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Multi-lineage differentiation of HCCs and chemotactic migration A) Chondrogenic differentiation in pellet culture. Safranin-O/fast green staining displayed strong proteoglycan deposition. B) Osteogenic differentiation in monolayer cultured HCCs. Alizarin Red (dark red) staining showed massive calcium phosphate deposition. C) Adipogenic differentiation in monolayer HCCs. Only few cells were positive for Oil Red O staining. D) Migrating cells respond to HMGB1 stimulation. HCCs were strongly chemotactic to HMGB1, with significantly higher cell migration than treated with SF; HCCs also showed significantly higher migration than NCs upon HMGB1 stimulation. Numbers above the bars indicate P values for differences between each group; Bars show the mean ± 95% CI. (Scale bar represents 200 μm). Osteoarthritis and Cartilage 2014 22, 1318-1326DOI: (10.1016/j.joca.2014.07.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Functional difference of superficial and deep HCCs. A) Deep HCCs showed superior chondrogenic potential with stronger Safranin-O staining for proteoglycan on both day 7 (left) and day 21 (right), and also showed higher chondrogenic genes expression. B) Deep HCCs had stronger osteogenic ability than superficial HCCs, with more calcium phosphate deposition on Alizarin Red staining and osteogenic markers higher expression. C) Superficial HCCs displayed higher level of lubricin both for immunostaining and gene expression than deep HCCs. The error bars in each plot represent the 95% CI. Osteoarthritis and Cartilage 2014 22, 1318-1326DOI: (10.1016/j.joca.2014.07.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions