Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington

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Presentation transcript:

Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in Novel Advanced Materials and Processing with Applications in Biomedical, Electrical and Chemical Engineering

Objective Design localized and automated delivery system for microfluidic brain slice devices (μBSDs) Delivers chemicals/drugs and gases Automatically mixes oxygen Characterize delivery of chemicals through tissue Image taken from Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8, 1048-1055.

Motivation μBSDs allow in vitro study of: Neurogenesis Synaptogenesis Regeneration Protein expression Responses to physical trauma Image taken from http://www.koki.hu/cdnb/research/research.html Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180, 243-254.

Relevance Chemical Delivery Oxygen Delivery Automation Spatial resolution Current systems affect entire slice with drug Desire stimulation of specific areas Oxygen Delivery Automatic Mixing Saves money Pre-mixing is not required Automation Multi-tasking Image modified from http://www.imagingeconomics.com/issues/articles/MI_2006-08_01.asp

Oxygen Mixing and Delivery Experimental Set-Up Y Connector Output Tube Valves Gas Feed Lines: 0% and 21% Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor

Oxygen Mixing and Delivery Output

Oxygen Mixing and Delivery Graphical User Interface (GUI)

Delivery Characterization μBSD Design Reservoir T Channel Delivery Channel

Delivery Characterization Experimental Set-up DI Water Lines T Channel Valve μBSD Vacuum Line

Delivery Characterization No Tissue Delivery 10ms 15ms 20ms 25ms 2x5ms 3x5ms 4x5ms 5x5ms

Delivery Characterization Linear Relationships: No Tissue 860 Width (µm) 1320 Width (µm)

Delivery Characterization Linear Relationships: With Tissue Full Dose Chemical Delivery Maximum Intensities 10 20 30 Valve Open Time (ms) Intensity Intensity 2 Time (min) Pulsing Dose Chemical Delivery Maximum Intensities 2 4 6 Number of 5ms Pulses Intensity Intensity 2 Time (min)

Delivery Characterization GUI

Conclusions Oxygen Delivery Chemical Delivery Precise and accurate results Reliable oxygen switching Chemical Delivery Demonstrated linear relationship Area of effect: ~2mm x 2mm Selective and independent valve control

Acknowledgements REU Directors Research Advisor Mentors Funding NSF-REU DoD-ASSURE EEC-NSF Grant # 0755115 REU Directors Christos G. Takoudis, Ph.D. Greg Jursich, Ph.D. Research Advisor David Thomas Eddington, Ph.D. Mentors Alexander Blake, Ph.D. Gerardo Mauleon