Figure 1. An example of a thioviridamide-like molecule, thioalbamide, and inset, a proposed biochemical route to ... Figure 1. An example of a thioviridamide-like.

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Figure 1. An example of a thioviridamide-like molecule, thioalbamide, and inset, a proposed biochemical route to ... Figure 1. An example of a thioviridamide-like molecule, thioalbamide, and inset, a proposed biochemical route to thioamides. Thioamides are highlighted in blue and other post-translational modifications are colored red. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz192, https://doi.org/10.1093/nar/gkz192 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. RiPPER identification of putative precursor peptides Figure 2. RiPPER identification of putative precursor peptides. (A) Schematic of RiPPER workflow where a cluster is ... Figure 2. RiPPER identification of putative precursor peptides. (A) Schematic of RiPPER workflow where a cluster is identified based on a putative RiPP tailoring enzyme (RTE). (B) The 30 largest peptide similarity networks identified using RiPPER for peptides associated with tfuA-like genes in Actinobacteria. Red numbers indicate networks predicted to comprise of authentic precursor peptides (see Supplementary Table S1 and Figures S7-S20) and triangular nodes indicate peptides encoded on the opposite strand to the RTE gene. Additional color-coding of nodes reflects domains with a probable association with a biosynthetic gene cluster and includes putative precursor peptides (nitrile hydratase-like (8) and type-A lantibiotic) and other small proteins (PqqD-like proteins (54,55), acyl carrier proteins and regulatory proteins). Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz192, https://doi.org/10.1093/nar/gkz192 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 3. Thioviridamide-like precursor peptides Figure 3. Thioviridamide-like precursor peptides. (A) The precursor peptide network that includes both ... Figure 3. Thioviridamide-like precursor peptides. (A) The precursor peptide network that includes both thioviridamide-like precursor peptides (red nodes) and a related but uncharacterized family of precursor peptides from BGCs that are highly different to thioviridamide-like BGCs (blue nodes). Characterized compounds are listed with their respective nodess. (B) Comparative analysis of thioviridamide-like and non- thioviridamide-like BGCs from this network where related genes share the same color. See Supplementary Figure S7 for full BGC details. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz192, https://doi.org/10.1093/nar/gkz192 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 4. Examples of putative RiPP BGCs and associated TfuA phylogeny Figure 4. Examples of putative RiPP BGCs and associated TfuA phylogeny. A maximum likelihood tree (branch lengths ... Figure 4. Examples of putative RiPP BGCs and associated TfuA phylogeny. A maximum likelihood tree (branch lengths removed) of TfuA-like proteins is color-coded to indicate the relationship between TfuA-like proteins and the associated networks of putative precursor peptides. Representative BGCs are also shown, where grey genes indicate genetic features that are conserved across multiple BGCs within that family. Fully annotated BGCs are shown in Supplementary Figures S7–S20. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz192, https://doi.org/10.1093/nar/gkz192 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 5. Identification of the thiovarsolin family of RiPPs Figure 5. Identification of the thiovarsolin family of RiPPs. (A) The associated precursor peptide network. (B) BGCs ... Figure 5. Identification of the thiovarsolin family of RiPPs. (A) The associated precursor peptide network. (B) BGCs associated with each precursor peptide. The protein product of each var gene is listed at the top (HO = heme oxygenase; AmT = amidinotransferase; MFS = major facilitator superfamily) and genes common to multiple BGCs are color-coded by the predicted function of the protein product (see Supplementary Figure S17 for full details). (C) Putative repeating precursor peptides identified by similarity networking. The predicted leader peptide is aligned, while the repeat regions are highlighted. Underlined text indicates the partially conserved core peptide that the thiovarsolins derive from, and bold text indicates equivalent residues in the other precursor peptides. (D) Analysis of thiovarsolin production by S. coelicolorM1146-TARvar, which contains a 31.7 kb DNA fragment centered on the S. varsoviensis BGC. Base peak chromatograms of crude extracts of S. coelicolorM1146-TARvar and an empty vector negative control (pCAP03) are shown, with peaks corresponding to thiovarsolins A-D indicated. Thioamidation and dehydrogenation post-translational modifications are highlighted on the thiovarsolin structures. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz192, https://doi.org/10.1093/nar/gkz192 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 6. Mutational analysis of thiovarsolin biosynthesis Figure 6. Mutational analysis of thiovarsolin biosynthesis. Extracted ion chromatograms (EICs) are shown for each ... Figure 6. Mutational analysis of thiovarsolin biosynthesis. Extracted ion chromatograms (EICs) are shown for each thiovarsolin (A = m/z 399.18, B = m/z 401.20, C = m/z 385.16, D = m/z 387.18). M1146 pCAP03 indicates the empty plasmid control, while each Δvar mutation was made in the TARvar construct and expressed in S. coelicolor M1146. See text and Supplementary Figure S36 for details of varA*. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz192, https://doi.org/10.1093/nar/gkz192 The content of this slide may be subject to copyright: please see the slide notes for details.