Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort.

Slides:

Advertisements

Similar presentations

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Advertisements

International Committee for Monitoring Assisted Reproductive Technology (ICMART) world report: assisted reproductive technology 2003 Karl G. Nygren,

Grace Younes, M.D., Togas Tulandi, M.D., M.H.C.M.

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Study of nucleation status in the second cell cycle of human embryo and its impact on implantation rate Jesus Aguilar, Ph.D., Irene Rubio, Ph.D., Elkin.

A giant oocyte in a cohort of retrieved oocytes: does it have any effect on the in vitro fertilization cycle outcome? Ronit Machtinger, M.D., Joseph.

Report of results obtained in 2,934 women using donor sperm: donor insemination versus in vitro fertilization according to indication Thamara Viloria,

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Successful pregnancy and delivery of two sets of monozygotic twins after intracytoplasmic sperm injection and embryo transfer: case report and literature.

Reporting in vitro fertilization cycles to the Society for Assisted Reproductive Technology database: where have all the cycles gone? David Kulak, M.D.,

Intrauterine injection of human chorionic gonadotropin before embryo transfer significantly improves the implantation and pregnancy rates in in vitro.

Effect of a very-low-calorie diet on in vitro fertilization outcomes

Successful delivery after the transfer of embryos obtained from a cohort of incompletely in vivo matured oocytes at retrieval time Patricia Fauque, M.D.,

What is the optimal follicular size before triggering ovulation in intrauterine insemination cycles with clomiphene citrate or letrozole? An analysis.

Zhi-Yong Yang, M.D., Ri-Cheng Chian, Ph.D. Fertility and Sterility

Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor, and cryopreserved cycles with the use of day.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Is frozen embryo transfer cycle associated with a significantly lower incidence of ectopic pregnancy? An analysis of more than 30,000 cycles Bo Huang,

Reducing multiples: a mathematical formula that accurately predicts rates of singletons, twins, and higher-order multiples in women undergoing in vitro.

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos Alberto Tejera, Ph.D., Javier Herrero, Ph.D.,

Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis Pravin T. Goud, M.D., Ph.D., Anuradha P.

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Management of the first in vitro fertilization cycle for unexplained infertility: a cost- effectiveness analysis of split in vitro fertilization-intracytoplasmic.

The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility.

Improvements achieved in an oocyte donation program over a 10-year period: sequential increase in implantation and pregnancy rates and decrease in high-order.

Automatic time-lapse instrument is superior to single-point morphology observation for selecting viable embryos: retrospective study in oocyte donation

Association of the very early rise of human chorionic gonadotropin with adverse outcomes in singleton pregnancies after in vitro fertilization Christopher.

Effect of the male factor on the clinical outcome of intracytoplasmic sperm injection combined with preimplantation aneuploidy testing: observational.

Looking back: egg donors' retrospective evaluations of their motivations, expectations, and experiences during their first donation cycle Nancy J. Kenney,

Does the father matter? The association between the periconceptional paternal folate status and embryonic growth Jeffrey Hoek, M.D., Maria P.H. Koster,

Use of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with cryptozoospermia: a meta-analysis Nikita Abhyankar, M.B.ChB.,

Programming the endometrium for deferred transfer of cryopreserved embryos: hormone replacement versus modified natural cycles Eva R. Groenewoud, M.D.,

What are patients doing with their mosaic embryos

Obesity reduces uterine receptivity: clinical experience from 9,587 first cycles of ovum donation with normal weight donors José Bellver, M.D., Antonio.

Normal birth after single-embryo transfer in a patient with excessive polypronuclear zygote formation following in vitro fertilization and intracytoplasmic.

Impact of oocyte donation on perinatal outcome in twin pregnancies

Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis Aylin Pelin Cil, M.D., Heejung Bang, Ph.D.,

Ongoing twin pregnancy after rescue intracytoplasmic sperm injection of unfertilized abnormal oocytes Navid Esfandiari, D.V.M., Ph.D., H.C.L.D., E. Anne.

Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization.

Cumulative newborn rates increase with the total number of transferred embryos according to an analysis of 15,792 ovum donation cycles Nicolás Garrido,

A randomized clinical trial to evaluate the effect of granulocyte-macrophage colony- stimulating factor (GM-CSF) in embryo culture medium for in vitro.

Anti-Müllerian hormone and inhibin B as predictors of pregnancy after treatment by in vitro fertilization/intracytoplasmic sperm injection Dorothea M.

Ana Cobo, Ph. D. , Aila Coello, Ph. D. , Jose Remohí, M. D

Number of good quality embryos on day 3 is predictive for both pregnancy and implantation rates in in vitro fertilization/intracytoplasmic sperm injection.

Poor sperm quality affects clinical outcomes of intracytoplasmic sperm injection in fresh and subsequent frozen–thawed cycles: potential paternal effects.

Efficacy and safety of human chorionic gonadotropin for follicular phase stimulation in assisted reproduction: a systematic review and meta-analysis

Effect of treatment of a previous ectopic pregnancy on in vitro fertilization– intracytoplasmic sperm injection outcomes: a retrospective cohort study

Storage of human oocytes in the vapor phase of nitrogen

Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success J. Ryan Martin, M.D., Jason G. Bromer,

Tong Du, M. S. , Hong Chen, M. D. , Rong Fu, M. S. , Qiuju Chen, Ph. D

José Bellver, M. D. , Yanira Ayllón, M. D. , Marcos Ferrando, M. D

Zsolt P. Nagy, M. D. , Ph. D. , Laura F. Rienzi, Ph. D. , Filippo M

Recai Pabuccu, M. D. , Gogsen Onalan, M. D. , Umit Goktolga, M. D

Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome Mehdi Benchaib, M.D., Ph.D., Jacqueline.

Use of Utrogestan during controlled ovarian hyperstimulation in normally ovulating women undergoing in vitro fertilization or intracytoplasmic sperm injection.

Lapse in embryo transfer training does not negatively affect clinical pregnancy rates for reproductive endocrinology and infertility fellows Jessica.

Eric S. Surrey, M. D. , Debra A. Minjarez, M. D. , John M. Stevens, B

Genetic evaluation procedures at sperm banks in the United States

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Irene Rubio, Ph. D. , Reidun Kuhlmann, Inge Agerholm, Ph. D

Parabolic trend in endometrial thickness at embryo transfer in in vitro fertilization/intracytoplasmic sperm injection cases with clinical pregnancy evidence

Saad Elzanaty, M.D., Ph.D., Johan Malm, M.D., Ph.D.

Cumulative live birth rates in cohorts of patients treated with in vitro fertilization or intracytoplasmic sperm injection Cynthia Witsenburg, M.D.,

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Ongoing pregnancy rates in intrauterine insemination are affected by late follicular- phase progesterone levels Antonio Requena, M.D., María Cruz, Ph.D.,

Preimplantation genetic screening: what is the clinical efficiency?

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Mohamed Fawzy, M. D. , Mai Emad, B. Sc. , Mohamed Y. AbdelRahman, M. D

Numerical chromosome anomalies detected in paternally derived pronuclei of tripronuclear zygotes after intracytoplasmic sperm injection Ervin Macas,

Presentation transcript:

Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort study Marcos Meseguer, Ph.D., Irene Rubio, Ph.D., Maria Cruz, Ph.D., Natalia Basile, Ph.D., Julian Marcos, Ph.D., Antonio Requena, M.D. Fertility and Sterility Volume 98, Issue 6, Pages 1481-1489.e10 (December 2012) DOI: 10.1016/j.fertnstert.2012.08.016 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Differences in clinical pregnancy rate for all intracytoplasmic sperm injection (ICSI) treatments for the period in which the time-lapse monitoring system (TMS) was used in the different clinics: relative improvement in clinical pregnancy rate for all ICSI treatments using TMS versus standard incubator (blue bars, left-hand y-axis), and the number of TMS treatment cycles in each clinic (diamonds, right-hand y-axis). The average value is weighted by the number of TMS cycles in each clinic. Fertility and Sterility 2012 98, 1481-1489.e10DOI: (10.1016/j.fertnstert.2012.08.016) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Graphic representation of the two main confounding factors. (A) Oocyte donation: significantly higher pregnancy rate for donation cycles versus autologous cycles and significantly higher abundance of donation cycles in the time-lapse monitoring system. (B) Day of transfer: significantly higher pregnancy rate for day 5 transfer versus day 3 transfer and significantly lower abundance of day 5 transfers in the time-lapse monitoring system. Fertility and Sterility 2012 98, 1481-1489.e10DOI: (10.1016/j.fertnstert.2012.08.016) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Evaluation of possible confounding factors to be included in logistic regression model. Only the parameters in the green quadrant are characterized by: 1) a significant effect on pregnancy rate; and 2) a difference in the incidence of each parameter between the time-lapse monitoring system (TMS) and the standard incubator (SI). Those green parameters are categorized as confounding factors and therefore included in the model. The remaining parameters did not present any effect on pregnancy rate (orange) or did not present any different incidence between TMS and SI (red). Fertility and Sterility 2012 98, 1481-1489.e10DOI: (10.1016/j.fertnstert.2012.08.016) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Simulated development of accumulated clinical pregnancy rate for three sample clinics. The red line indicates the average pregnancy rate encountered in the standard incubator in the present study (44.9%). The green line is the expected average pregnancy rate assuming a 20.1% relative increase, i.e., 53.9%. The outcome of different clinics would vary initially, but after several hundred cycles the results converge near the expected clinical pregnancy rate. TMS = time-lapse monitoring system. Fertility and Sterility 2012 98, 1481-1489.e10DOI: (10.1016/j.fertnstert.2012.08.016) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Expected percentage of clinics with a given improvement after a given number of cycles based on a binomial distribution of pregnancy outcome. The clinical pregnancy rate for the standard incubator in the present study was 44.9%. The expectations assume an average pregnancy rate relative increase of 20.1%, i.e., to 53.9%. The different colored areas represent the proportions of clinics that are expected to experience a improvements in pregnancy success rate. With limited number of cycles it is to be expected that many clinics would experience an inferior or an unsustainable elevated result. Fertility and Sterility 2012 98, 1481-1489.e10DOI: (10.1016/j.fertnstert.2012.08.016) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 Simulated development of running average of clinical pregnancy rate for the last 50 cycles for the three sample clinics in Supplemental Figure 1. The red line indicates the average pregnancy rate encountered in the standard incubator in the present study (44.9%). The green line is the expected average pregnancy rate assuming a 20.1% relative increase, i.e., to 53.9%. Given the probability of success, it is expected that all clinics would occasionally experience extended periods with inferior results. TMS = time-lapse monitoring system. Fertility and Sterility 2012 98, 1481-1489.e10DOI: (10.1016/j.fertnstert.2012.08.016) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions