Toll-Like Receptor 3 Increases Allergic and Irritant Contact Dermatitis Naomi Nakamura, Risa Tamagawa-Mineoka, Mayumi Ueta, Shigeru Kinoshita, Norito.

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Presentation transcript:

Toll-Like Receptor 3 Increases Allergic and Irritant Contact Dermatitis Naomi Nakamura, Risa Tamagawa-Mineoka, Mayumi Ueta, Shigeru Kinoshita, Norito Katoh Journal of Investigative Dermatology Volume 135, Issue 2, Pages 411-417 (February 2015) DOI: 10.1038/jid.2014.402 Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Impaired CHS response in Tlr3 KO mice. (a) Change in ear-swelling responses of wild-type (WT; n=12), Tlr3 knockout (KO; n=6), and TLR3 transgenic (Tg; n=11) mice. (b) Histologic examination of ear skin. Hematoxylin and eosin. Original magnification × 100. Bar=50 μm. (c) Numbers of cells infiltrated in the dermis. Data are expressed as the means±SD. **P<0.01. CHS, contact hypersensitivity. Journal of Investigative Dermatology 2015 135, 411-417DOI: (10.1038/jid.2014.402) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 TLR3 is required for the elicitation phase of CHS, but not the sensitization phase. Lymph node cells were prepared from sensitized wild-type (WT) or Tlr3 knockout (KO) donors and adoptively transferred into naive WT or Tlr3 KO recipients (n=4–9 per group). (a) Change in ear-swelling responses at 24 hours after challenge. (b) Numbers of cells infiltrated in the dermis. Data are expressed as the means±SD. *P<0.05; **P<0.01. CHS, contact hypersensitivity; TNCB, 2,4,6-trinitro-1-chlorobenzene. Journal of Investigative Dermatology 2015 135, 411-417DOI: (10.1038/jid.2014.402) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Impaired ICD response in Tlr3 KO mice. (a) Changes in ear-swelling responses of wild-type (WT; n=8), Tlr3 knockout (KO; n=8), and TLR3 transgenic (Tg; n=9) mice. (b) Histologic examination of ear skin. Hematoxylin and eosin. Original magnification × 100. Bar=50 μm. (c) Numbers of cells infiltrated in the dermis. Data are expressed as the means±SD. *P<0.05; **P<0.01. ICD, irritant contact dermatitis. Journal of Investigative Dermatology 2015 135, 411-417DOI: (10.1038/jid.2014.402) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Comparison of DC and lymphocyte function between WT and Tlr3 KO mice. (a) Numbers and percentages among total lymph node cells of FITC+ CD11c+ DCs, FITC+ CD86+, and FITC+ MHC class II+ were analyzed by flow cytometry 24 hours after FITC application (n=3 per group). (b) CD11c+ DCs prepared from spleen of WT or Tlr3 KO mice were incubated with CFSE-labeled lymph node cells of C57BL/6 mice for 7 days (n=3 per group). (c) CFSE-labeled lymph node cells of WT or Tlr3 KO mice were incubated with allogeneic CD11c+ DCs prepared from spleen of C57BL/6 mice for 7 days (n=3 per group). The percentage of proliferating cells is shown. CFSE, carboxyfluorescein succinimidyl ester; DC, dendritic cell; KO, knockout; WT, wild-type. Journal of Investigative Dermatology 2015 135, 411-417DOI: (10.1038/jid.2014.402) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Production of cytokines and chemokines in TNCB-induced CHS. Quantitative real-time PCR analysis of IL-1α, IL-1β, TNF-α, KC, IP-10, and RANTES mRNA expression was performed in the whole ear at 24 hours after challenge (n=9 per group). Data are expressed as the means±SD. *P<0.05; **P<0.01. CHS, contact hypersensitivity; IP-10, interferon-γ-inducible protein 10; KC, keratinocyte chemoattractant; KO, knockout; RANTES, regulated on activation, normally T-cell expressed and secreted; TNCB, 2,4,6-trinitro-1-chlorobenzene; TNF, tumor necrosis factor; WT, wild-type. Journal of Investigative Dermatology 2015 135, 411-417DOI: (10.1038/jid.2014.402) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions