Inquiry into Life Eleventh Edition Sylvia S. Mader Chapter 3 Lecture Outline Prepared by: Wendy Vermillion Columbus State Community College Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

3.1 Cellular level of organization Cells are the smallest unit of life Exhibit all characteristics of life All cells are highly organized Many cells become specialized for complex functions Cell theory All living things are composed of cells Cells are the functional and structural units of organisms All cells are derived from previously existing cells Discovery of cells Antonie van Leeuwenhoek- invented the light microscope Robert Hooke- first observed cells in cork Schleiden and Schwann-proposed cell theory

Cellular level of organization, cont’d. Cell size Most cells are smaller than 1 mm in diameter Surface/volume ratio determines cell size Volume increases as the cube of the radius while surface area increases by the square of the radius Therefore small cells have a greater surface/volume ratio than larger cells Nutrients from the environment must cross the surface of the cell to enter Cells must be small in order for the surface area to be adequate to supply nutrients

3.2 Eukaryotic cells Eukaryotic cells have a membrane-bound nucleus Nucleus contains genetic material All plant and animal cells are eukaryotic Plasma membrane-outer boundary of cell Phospholipid bilayer-note arrangement of hydrophilic and hydrophobic ends Embedded proteins Associated glycolipids and cholesterol

Eukaryotic cells, cont’d. Functions of the cell membrane Functions as a barrier between the cell and its environment Plays a role in regulation of transport of substances into and out of the cell Contains receptors that determine how a cell will respond to stimuli in the environment Contains proteins that are important in immune responses It is a very dynamic, fluid structure Plant cells have an outer cell wall in addition to the plasma membrane which is composed of cellulose for rigidity Some plant cells have a secondary cell wall which is composed of lignin

Eukaryotic cells, cont’d. Organelles-subcellular structures which perform specific life functions for the cell Many organelles are found in both animal and plant cells Some are found exclusively in plants or animals Plants- chloroplasts, central vacuole Animals-centrioles

Animal cell anatomy Fig. 3.2

Plant cell anatomy Fig. 3.3

Eukaryotic cells, cont’d. Nucleus Contains the genetic material DNA Nucleoplasm-semifluid within nucleus Chromatin-threadlike DNA which has a grainy appearance Nucleolus-dark regions of chromatin which produce rRNA which composes ribosomes Nuclear membrane- double layered, surrounds nucleus and has large pores

The nucleus and nuclear envelope Fig 3.4

Eukaryotic cells, cont’d. Ribosomes- site of protein assembly Composed of RNA subunits Exist either as free ribosomes or bound to endoplasmic reticulum Endomembrane system- Includes nuclear membrane, endoplasmic reticulum (er), Golgi apparatus, and vesicles

Eukaryotic cells, cont’d. Endomembrane system cont’d. Rough endoplasmic reticulum (rer) Complex system of sacs and channels Has attached ribosomes Serves as site of assembly of proteins for export Assembled proteins enter channels for processing Addition of sugar chains to form glycoproteins Released in vesicles Smooth endoplasmic reticulum (ser) Synthesizes lipid products such as phospholipids and steroids

The endoplasmic reticulum Fig 3.5

Eukaryotic cells, cont’d. Golgi apparatus packaging and processing center for cell products Receives the vesicles from er Vesicles fuse with Golgi and products are released inside Further modification of glyoproteins occurs Products are packaged into secretory vesicles and released to the cell membrane Golgi also produces lysosomes-protein containing vesicles within cells

The endomembrane system Fig 3.6

Eukaryotic cells, cont’d. Lysosomes Contain hydrolytic enzymes Fuse with vesicles from cell membrane containing macromolecules Digestion occurs and nutrients released to cell Also may be involved in programmed cell death-”suicide sacs” Lysosomal membranes in old or damaged cells rupture and enzymes digest the cell

Peroxisomes Fig 3.7

Eukaryotic cells, cont’d. Energy related organelles Chloroplasts- site of photosynthesis Capture light energy and convert it to chemical energy in the form of food molecules Double outer membrane surrounds fluid-filled stroma Membranous system of thylakoids Regions of stacked thylakoids are called grana

Chloroplast structure Fig 3.8

Eukaryotic cells, cont’d. Mitochondria-another energy related organelle Site of aerobic cell respiration-production of ATP Outer double membrane surrounds fluid-filled matrix Inner folded membrane-folds are called cristae Cristae provide increased surface area for the production of ATP

Mitochondrion structure Fig. 3.9

Eukaryotic cells, cont’d. Interrelationship between mitochondria and chloroplasts Chloroplasts convert light energy to chemical energy in the form of organic food molecules (carbohydrates) which can be used by all organisms in cell respiration to make ATP Only plants and algae have chloroplasts All cells, plant and animal, contain mitochondria Photosynthesis can be summarized by the following: Light energy + carbon dioxide + water  carbohydrate + oxygen Cell respiration can be summarized below: Carbohydrate + oxygen  carbon dioxide + water + energy (ATP) It can be seen that the chemicals required by cell respiration are produced in photosynthesis, and vice versa

Eukaryotic cells, cont’d. The cytoskeleton Maintains cell shape Allows cells and organelles to move Components include actin filaments, intermediate filaments, and microtubules Actin filaments interact with motor molecule myosin in muscles Intermediate filaments support the nuclear membrane Microtubules protrude from the centrosome and form centrioles, cilia, and flagella

The cytoskeleton Fig. 3.10 Fig 3.11

Eukaryotic cells, cont’d. Centrioles Short tubules with 9+0 pattern of microtubule triplets In animal cells, centrosome is composed of 2 centrioles Believed to be involved in microtubule formation including mitotic spindle Cilia and flagella Hair-like projections, cilia generally multiple and flagella single or double 9+2 pattern of microtubules Whip-like action

Actin and intermediate filaments Fig 3.12 Fig 3.13

Centrioles Fig. 3.14

Structure of cilia and flagella Fig. 3.15

3.3 Prokaryotic cells Archae and bacteria are prokaryotes Lack a nucleus Smaller than eukaryotes Prokaryotic cell structures Cell wall-contains peptidoglycans Capsule present in some Plasma membrane Nucleoid-region which contains a single chromosome Ribosomes Thylakoids-in photosynthetic cyanobacteria

Bacterial cells Fig 3.16

3.4 Evolution of eukaryotic cells Endosymbiont hypothesis-prokaryotes engulfed by larger cells Chloroplasts and mitochondria resemble bacteria Both have double membrane-outer could be from vesicle, inner from original cell membrane Contain DNA and are self-replicative Contain their own ribosomes RNA base sequences suggest relationship with bacteria

Evolution of eukaryotic cells Fig 3.17