Development of a Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of the JAK2 V617F Mutation Elizabeth C. Wolstencroft, Katy.

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Development of a Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of the JAK2 V617F Mutation Elizabeth C. Wolstencroft, Katy Hanlon, Lorna W. Harries, Graham R. Standen, Alexander Sternberg, Sian Ellard The Journal of Molecular Diagnostics Volume 9, Issue 1, Pages 42-46 (February 2007) DOI: 10.2353/jmoldx.2007.060083 Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Standard curves for the mutant and wild-type JAK2 probes generated by 1:2 serial dilution of DNA. The Journal of Molecular Diagnostics 2007 9, 42-46DOI: (10.2353/jmoldx.2007.060083) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 A: Mixing curve showing limit of detection of the real-time PCR assay. 1, 100% mutant; 2, 10% mutant; 3, 7.5% mutant; 4, 5% mutant; 5, 2.5% mutant; 6, 1% mutant; 7, 0.5% mutant; and 8, 100% wild type. B: The nonspecific hybridization of the mutant probe to wild-type sequence can easily be distinguished from genuine low positives. The Journal of Molecular Diagnostics 2007 9, 42-46DOI: (10.2353/jmoldx.2007.060083) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions