Molecular characterization of dog albumin as a cross-reactive allergen

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Presentation transcript:

Molecular characterization of dog albumin as a cross-reactive allergen Susanne Spitzauer, MDa, Christian Schweiger, MDa, Wolfgang R. Sperr, MDb, Budhi Pandjaitana, Peter Valent, MDb, Sonja Mühla, Christof Ebner, MDc, Otto Scheiner, PhDc, Dietrich Kraft, MDc, Helmut Rumpold, MDa, Rudolf Valenta, MDc Journal of Allergy and Clinical Immunology Volume 93, Issue 3, Pages 614-627 (March 1994) DOI: 10.1016/S0091-6749(94)70073-7 Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 1 Complementary DNA and deduced amino acid sequence of clone 54c. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 2A Alignment of the deduced amino acid sequence of clone 54c with albumin sequences. The deduced amino acid sequence of clone 54c coding for a dog albumin fragment is compared with albumin sequences; human (82.6%), pig (81.8%), cattle (77.3%), sheep (78.8%), mouse (75.8%), and rat (76.2%). Vertical bars represent identical amino acids. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 2B See legend to FIG. 2A. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 2C See legend to FIG. 2A. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 3A IgE reactivity of selected patients allergic to albumin with dog hair/dander extract. IgE Immunoblot with dog hair/danDer proteins that were blotted to nitro cellulose. Six patients with IgE reactivity to dog albumin had been selected and were compared with a human serum pool prepared from nonallergic patients (N). P represents the buffer control. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 3B IgE reactivity of patients allergic to dogs with different albumins. The same group of individuals (patient 1 to 6) as in Fig. 3A was tested for IgE reactivity to nitrocellulose-blotted purified albumins from dog, cat, mouse, and rat. NP represents a serum pool from nonallergic patients and P represents the buffer control. IgE binding to the various albumins is differently pronounced. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 4 Inhibition of IgE binding to different albumins with dog albumin. Purified albumins from dog, mouse, rat, and cat were transferred to nitrocellulose. Strips were incubated with serum from a patient allergic to dog albumin. In lanes 1, 3, 5, and 7 the serum was preincubated with purified dog albumin and in lanes 2, 4, 6, and 8 the serum was preincubated with timothy grass pollen extracts. IgE binding to dog albumin and the other mammalian albumins was significantly reduced on preincubation with dog albumin. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 5 Dose dependency of the histamine release from basophils of a patient allergic to albumin (A) and a control subject without albumin-specific IgE (B). Granulocytes of the donors (A, B) were incubated with various concentrations of albumin from dog, rat, mouse, chicken, buffer, or monoclonal anti-IgE antibody. Histamine release in the cell-free supernatants was determined by radioimmunoassay. Percentage of total histamine is displayed and reflects means of triplicate determinations. The standard deviation is displayed for each value with the exception of the anti-IgE control, where the maximum release is indicated with an asterisk. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 6 Testing of recombinant phage with serum IgE from a patient allergic to dog albumin. A, Nitrocellulose filters I and II containing plaque lifts of phage, which had been induced to produce recombinant proteins, were tested with serum IgE from a dog albumin–allergic patient who also displays IgE reactivity to the major birch pollen allergen, Bet v I. B, Recombinant phages, which had been isolated from a dog salivary gland cDNA library in filters I and II, are designated with various numbers: nKo represents nonrecombinant lambda gt11 phage and Bet v I represents the phage expressing the major birch pollen allergen Bet v I as a positive control. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 7 Sequences of oligonucleotides complementary to different portions of the dog albumin cDNA. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 8 Hybridization of albumin-specific oligonucleotides with cDNA clones coding for dog allergens. A, Plaque lifts of phages, which had been isolated with serum IgE from patients allergic to dogs, nonrecombinant lambda gt11 phage (nKo), and phages that contain cDNA from clone 54c coding for dog albumin (pKo) were hybridized with synthetic oligonucleotides complementary to different portions of the albumin cDNA (A). B, Localization of the clones on the nitrocellulose filters. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 8 Hybridization of albumin-specific oligonucleotides with cDNA clones coding for dog allergens. A, Plaque lifts of phages, which had been isolated with serum IgE from patients allergic to dogs, nonrecombinant lambda gt11 phage (nKo), and phages that contain cDNA from clone 54c coding for dog albumin (pKo) were hybridized with synthetic oligonucleotides complementary to different portions of the albumin cDNA (A). B, Localization of the clones on the nitrocellulose filters. Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 9 Comparison of the IgE binding to natural dog hair/danDer proteins and to recombinant albumin fragments. All patients with IgE binding to dog albumin in natural dog hair/dander extracts (A) show comparable reactivity with a recombinant dog albumin fragment expressed in E. coli as β-galactosidase fusion protein (B) at around 150 kd. No IgE binding is observed with protein extracts prepared from lysogenic E. coli Y1089 infected with nonrecombinant lambda gt11 phage in the 150 kd region-negative control (C). Journal of Allergy and Clinical Immunology 1994 93, 614-627DOI: (10.1016/S0091-6749(94)70073-7) Copyright © 1994 Mosby, Inc. Terms and Conditions