Introduction to Gel Electrophoresis

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Presentation transcript:

Introduction to Gel Electrophoresis

What do you remember about DNA?

Gel Electrophoresis Gel Electrophoresis - a method for separation and analysis of DNA/protein

Separation of Molecules Separation of molecules is based on size and charge

Purpose Studying evolutionary relationships Forensics: DNA Fingerprinting Paternity Testing

How Does it Work? POWER SUPPLY SAMPLE WELLS ELECTROPHORESIS TANK + POSITIVE ELECTRODE DIRECTION OF MOVEMENT -NEGATIVE Samples are placed in a gel, which is placed in a solution that conducts electricity

Step 1 Prepare agarose gel

Step 2 Prepare DNA samples

Step 3 Load samples into gel

Step 4 Connect gel to electric current

Step 5 Analyze DNA pattern

YouTube Gel Electrophoresis