Invasion process of induced deep nodular endometriosis in an experimental baboon model: similarities with collective cell migration? Olivier Donnez,

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Invasion process of induced deep nodular endometriosis in an experimental baboon model: similarities with collective cell migration? Olivier Donnez, M.D., Ph.D., Renan Orellana, Ph.D., Olivier Van Kerk, B.Sc., Jean-Paul Dehoux, D.V.M., Ph.D., Jacques Donnez, M.D., Ph.D., Marie-Madeleine Dolmans, M.D., Ph.D. Fertility and Sterility Volume 104, Issue 2, Pages 491-497.e2 (August 2015) DOI: 10.1016/j.fertnstert.2015.05.011 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Gland thickness measurement. Graphic representation of the measurement of gland thickness (μm) in the noninvasive group (n = 34 glands), invasive center group (n = 48 glands), and invasive front group (n = 29 glands). ***P<.01. Fertility and Sterility 2015 104, 491-497.e2DOI: (10.1016/j.fertnstert.2015.05.011) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Ki67 immunostaining of (A) a noninvasive lesion (n = 56 glands), (B) the invasive center (n = 82 glands), and (C) invasion front glands (n = 40 glands). (D) Graphic representation of one-way analysis of variance. *P<.05; **P<.01. Fertility and Sterility 2015 104, 491-497.e2DOI: (10.1016/j.fertnstert.2015.05.011) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 3 E-Cadherin immunostaining of (A) a noninvasive lesion (n = 56 glands), (B) the invasive center (n = 84 glands), and (C) invasion front glands (n = 44 glands). (D) Graphic representation of one-way analysis of variance. ***P<.001. Fertility and Sterility 2015 104, 491-497.e2DOI: (10.1016/j.fertnstert.2015.05.011) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 4 β-Catenin immunostaining of (A) a noninvasive lesion (n = 42 glands), (B) the invasive center (n = 67 glands), and (C) invasion front glands (n = 54 glands). (D) Graphic representation of one-way analysis of variance. ***P<.001. Fertility and Sterility 2015 104, 491-497.e2DOI: (10.1016/j.fertnstert.2015.05.011) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Induced nodular lesions recovered from nine baboons (n = 20 glands). (A) Microscopic view of an invasive lesion (n = 11/20 glands; hematoxylin and eosin staining). (B) Microscopic view of a noninvasive lesion (n = 9/20 glands; hematoxylin and eosin staining). White dotted line: center of the lesion. Black arrows: invasion front glands. Fertility and Sterility 2015 104, 491-497.e2DOI: (10.1016/j.fertnstert.2015.05.011) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Correlation analysis between gland thickness and mitotic activity. Graphic representation of linear regression analysis (n = 109 glands; P<.01; r2 = 0.3356). Fertility and Sterility 2015 104, 491-497.e2DOI: (10.1016/j.fertnstert.2015.05.011) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions