Paola B. Navas, Alicia B. Motta, Ph. D. , María B. Hapon, Ph. D

Slides:

Advertisements

Similar presentations

The overexpression of cyclo-oxygenase-2 in chronic periodontitis

Advertisements

Raloxifene⁎ increases proliferation of human endothelial cells in association with increased gene expression of cyclins A and B1 Pilar J. Oviedo, B.Sc.,

Human chorionic gonadotropin controls luteal vascular permeability via vascular endothelial growth factor by down-regulation of a cascade of adhesion.

Sphingosine pathway deregulation in endometriotic tissues

Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls Hong Zhang,

Association of abdominal obesity, insulin resistance, and oxidative stress in adipose tissue in women with polycystic ovary syndrome Li Chen, M.S., Wen.

Augmented epithelial multidrug resistance–associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A4 Ilaria Gori, Ph.D., Yoima.

Abnormally up-regulated cystic fibrosis transmembrane conductance regulator expression and uterine fluid accumulation contribute to Chlamydia trachomatis-induced.

Differential expression of G-protein-coupled estrogen receptor-30 in human myometrial and uterine leiomyoma smooth muscle Ruijuan Tian, M.Sc., Zengyong.

The CB2 cannabinoid receptor regulates human sperm cell motility

Preeclampsia is associated with a deficiency of lipoxin A4, an endogenous anti- inflammatory mediator Zhangye Xu, M.D., Feng Zhao, M.M., Feng Lin, M.M.,

Effective treatment of experimental human endometrial cancers with targeted cytotoxic luteinizing hormone–releasing hormone analogues AN-152 and AN-207

Fertility and Sterility

Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells Christine Chabrolle, M.D., Lucie.

Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition Monica Faut, Evelin Mariel Elia,

Luteal-phase ovarian stimulation is feasible for producing competent oocytes in women undergoing in vitro fertilization/intracytoplasmic sperm injection.

2-Methoxyestradiol causes functional repression of transforming growth factor β3 signaling by ameliorating Smad and non-Smad signaling pathways in immortalized.

Hsien-An Pan, M. D. , Ph. D. , Yeu-Chin Lee, M. Sc. , Yen-Ni Teng, Ph

Dennis J. Hand, Ph. D. , Vanessa L. Short, Ph. D. , M. P. H. , Diane J

Xian-Hua Lin, M. D. , Miao-E. Liu, M. B. , Hai-Yan Xu, M. B

Direct effect of macrophage migration inhibitory factor on sperm function: possible involvement in endometriosis-associated infertility Cédric Carli,

Expression and localization of transcription factor Ets-1 in the rat ovary during the estrous cycle and pregnancy Xiaohui Xiao, M.S., Aiqin Liu, Ph.D.,

Modulation of the BCL-2/BAX ratio by interferon-γ and hypoxia in human peritoneal and adhesion fibroblasts Ghassan M. Saed, Ph.D., Zhongliang Jiang,

Marta Busnelli, Ph. D. , Valeria Rimoldi, Ph. D. , Paola Viganò, Ph. D

Julie L. V. Shaw, Ph. D. , Fiona C. Denison, M. B. , Ch. B. , M. D

Maternal protein-energy and energy-restricted diets during lactation possibly could program folliculogenesis and the ovarian expression of leptin and.

Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients Chun-Na An, M.S., Hui Jiang, M.D., Qi Wang, M.S.,

Estrogen receptor β regulates endometriotic cell survival through serum and glucocorticoid–regulated kinase activation Diana Monsivais, Ph.D., Matthew.

Endometrial stromal progesterone receptor-A/progesterone receptor-B ratio: no difference between women with and without endometriosis Davide Gentilini,

Medical treatment of ectopic pregnancy: a committee opinion

Derek K. Lobb, Ph.D. Fertility and Sterility

Clomiphene citrate potentiates the adverse effects of estrogen on rat testis and down- regulates the expression of steroidogenic enzyme genes Shilpa Bharti,

Peritoneal fluid macrophages in endometriosis: correlation between the expression of estrogen receptors and inflammation Paola Montagna, B.Sc., Silvia.

Nieves María Gabrielli, M. Sc. , María Florencia Veiga, Ph. D

Alternation of cytosolic carbonic anhydrase isoenzymes during deciduomatal development in pregnant mice Whei-Ling Chiang, Ph.D., Jer-Yuh Liu, Ph.D.,

Oxidative stress: a key regulator of leiomyoma cell survival

Administration of visfatin during superovulation improves developmental competency of oocytes and fertility potential in aged female mice Kyoung-Hwa.

María Guillermina Bilbao, Ph. D. , María Paula Di Yorio, Ph. D

Kisspeptin stimulates progesterone secretion via the Erk1/2 mitogen-activated protein kinase signaling pathway in rat luteal cells Jing Peng, M.D., Min.

Cell plating density alters the ratio of estrogenic to progestagenic enzyme gene expression in cultured granulosa cells Valério M. Portela, D.V.M., Ph.D.,

Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality Xingqi Zhang, Ph.D., Nadereh Jafari, Ph.D.,

Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility Sara Correia, M.S.,

Coadministration of dextromethorphan during pregnancy and throughout lactation prevents morphine-induced hyperprolactinemia in female rats Ling-Yi Wu,

Estrogen receptor β agonist diarylpropionitrile inhibits lipopolysaccharide-induced regulated on activation normal T cell expressed and secreted (RANTES)

Eszter Ducza, Ph. D. , Róbert Gáspár, Ph. D. , Attila Mihályi, Ph. D

Link between metformin and the peroxisome proliferator-activated receptor γ pathway in the uterine tissue of hyperandrogenized prepubertal mice Evelin.

Padmasana Singh, Ph. D. , Amitabh Krishna, Ph. D

Testosterone dependent androgen receptor stabilization and activation of cell proliferation in primary human myometrial microvascular endothelial cells

Requirement of heart and neural crest derivatives–expressed transcript 2 during decidualization of human endometrial stromal cells in vitro Hisayuu Shindoh,

Single and multidose pharmacokinetic study of a vaginal micronized progesterone insert (Endometrin) compared with vaginal gel in healthy reproductive-aged.

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Aromatase expression in abdominal omental/visceral and subcutaneous fat depots: a comparison of pregnant and obese women Suman Rice, Ph.D., Bijal Patel,

Jieqiang Lv, M. D. , Xueqiong Zhu, M. D. , Ph. D

Antimüllerian hormone inhibits follicle-stimulating hormone-induced adenylyl cyclase activation, aromatase expression, and estradiol production in human.

Hypoxia regulates iNOS expression in human normal peritoneal and adhesion fibroblasts through nuclear factor kappa B activation mechanism Zhong L. Jiang,

Introduction Fertility and Sterility

Hélio Sato, M. D. , Ph. D. , Naiara C. Nogueira-de-Souza, M. Sc. , Ph

Müllerian-inhibiting substance inhibits cytochrome P450 aromatase activity in human granulosa lutein cell culture Michael P. Grossman, M.D., Steven T.

Masaaki Iwahashi, M.D., Yasuteru Muragaki, M.D.

Histone deacetylase inhibitors down-regulate G-protein-coupled estrogen receptor and the GPER-antagonist G-15 inhibits proliferation in endometriotic.

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion.

Identification of differential gene expression in germinal vesicle vs

Hsien-An Pan, M. D. , Yue-Shan Lin, M. D. , Ko-Hung Lee, M. D

Estrogen and selective estrogen receptor modulators regulate vascular endothelial growth factor and soluble vascular endothelial growth factor receptor.

Susanna McReynolds, Ph. D. , Monika Dzieciatkowska, Ph. D

Treatment of heterotopic cervical pregnancy after in vitro fertilization–embryo transfer by using transvaginal ultrasound–guided aspiration and instillation.

An epigenetic disorder may cause aberrant expression of aromatase gene in endometriotic stromal cells Masao Izawa, Ph.D., Tasuku Harada, M.D., Fuminori.

Leptin role in the rat prostate ventral lobe

Anitha Pankajakshy, M.Sc., M.Phil., Indira Madambath, Ph.D.

Presentation transcript:

Hyperthyroidism advances luteolysis in the pregnant rat through changes in prostaglandin balance Paola B. Navas, Alicia B. Motta, Ph.D., María B. Hapon, Ph.D., Graciela A. Jahn, Ph.D. Fertility and Sterility Volume 96, Issue 4, Pages 1008-1014.e1 (October 2011) DOI: 10.1016/j.fertnstert.2011.07.010 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Effect of T4 treatment (A) on serum levels of T3, T4, TSH, PRL, E2, and P on days 19 (G19), 20 (G20), and 21 (G21) of pregnancy and (B) on corpus luteum (CL) number and weight on G21. Thyroxine was administered SC at a concentration of 0.25 mg/kg per day. Results are expressed as the mean ± SEM for groups of eight rats. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 and G20, respectively, in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test. Fertility and Sterility 2011 96, 1008-1014.e1DOI: (10.1016/j.fertnstert.2011.07.010) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Effect of T4 treatment on luteal messenger RNA abundances of enzymes related to P synthesis and degradation on days 19 (G19), 20 (G20), and 21 (G21). (A), Measurement by using reverse transcriptase–polymerase chain reaction (RT-PCR) of expression of steroidogenic acute regulatory protein (StAR), cytochrome P450scc, 3βHSD, 20αHSD, and S16. Ethidium bromide fluorescence photograph of the gel electrophoresis of the amplification products. The gel photographs were quantified using NIH Image software and expressed as arbitrary units. (B), Measurement by real-time RT-PCR of the expression of 20αHSD, relative to S16. (C), Western blot of 20αHSD protein on whole luteal protein extracts separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose and immunoblotted with specific 20αHSD (37 kDa) and α-tubulin (55 kDa) antisera. See Materials and Methods section for further details. Results are expressed as the mean ± SEM of groups of seven to eight rats. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 and G20, respectively, in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test. Fertility and Sterility 2011 96, 1008-1014.e1DOI: (10.1016/j.fertnstert.2011.07.010) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Effect of T4 treatment on luteal messenger RNA abundances of proteins involved in the regulation of luteal P synthesis and in prostaglandin synthesis and action on G19, G20, and G21. A, Measurement by using reverse transcriptase–polymerase chain reaction of expression of estrogen receptor alpha (ER-α), estrogen receptor beta (ER-β), PRL-R, PGF2α-R, iNOS, and S16. B, Expression of ER-α, ER-β, PRL-R, PGF2α-R, and iNOS relative to S16, respectively. The films were quantified using NIH Image software and expressed as arbitrary units. Results are expressed as the mean ± SEM of groups of seven to eight rats. See Materials and Methods section for further details. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test. Fertility and Sterility 2011 96, 1008-1014.e1DOI: (10.1016/j.fertnstert.2011.07.010) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Effect of T4 treatment on serum concentrations of PGF2α and PGE2 and luteal concentrations of P, PGF2α, and PGE2 on G19, G20, and G21. See Materials and Methods section for further details. Results are expressed as the means ± SEM for groups of seven to eight rats. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 and G20, respectively, in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test. Fertility and Sterility 2011 96, 1008-1014.e1DOI: (10.1016/j.fertnstert.2011.07.010) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions