Analysis of differentially expressed proteins involved in hand, foot and mouth disease and normal sera L. Deng, H.-L. Jia, C.-W. Liu, K.-H. Hu, G.-Q.

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Presentation transcript:

Analysis of differentially expressed proteins involved in hand, foot and mouth disease and normal sera L. Deng, H.-L. Jia, C.-W. Liu, K.-H. Hu, G.-Q. Yin, J.-W. Ye, C.-H. He, J.-H. Chen, Y.-P. Xie, R. Dang, L. Zhang, N.-Y. Liu, L. Zhu, L. Zhang Clinical Microbiology and Infection Volume 18, Issue 6, Pages E188-E196 (June 2012) DOI: 10.1111/j.1469-0691.2012.03836.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Analysis of hand, foot and mouth disease (HFMD) samples as revealed by 2-D DIGE analysis. Proteins extracted from HFMD and control samples were labelled with Cy3 and Cy5, respectively. An internal standard protein sample (a mixture of HFMD and control samples) was labelled with the Cy2 dye. The Cy3-labelled HFMD samples and the Cy5-labelled control samples were mixed and separated on a 2-D DIGE gel (IEF on pH 4-7 strips followed by 12.5% second dimension SDS-PAGE). The green spots represent down-regulated proteins, while the red spots represent up-regulated proteins in HFMD samples compared with the control samples. The yellow arrow represents the identified proteins that showed significantly differential expression in the HFMD and control samples. The number in the figure corresponds to that presented in Tables 1 and 2. (a) Mild HFMD vs control samples. (b) Severe HFMD vs control samples. Clinical Microbiology and Infection 2012 18, E188-E196DOI: (10.1111/j.1469-0691.2012.03836.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Classification analysis of proteins found in hand, foot and mouth disease (HFMD) samples. Categorization was based on information that was obtained from the online PANTHER classification system. (a) Classification of the identified proteins of HFMD samples according to the molecular function. (b) Classification of the identified proteins of HFMD samples according to the biological processes. (c) Classification of the identified proteins of HFMD samples according to the protein class. Clinical Microbiology and Infection 2012 18, E188-E196DOI: (10.1111/j.1469-0691.2012.03836.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 The network of protein-protein interactions of HFMD. Clinical Microbiology and Infection 2012 18, E188-E196DOI: (10.1111/j.1469-0691.2012.03836.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions