The Anti-C1s Antibody TNT003 Prevents Complement Activation in the Skin Induced by Bullous Pemphigoid Autoantibodies Anika Kasprick, Maike M. Holtsche,

Slides:

Advertisements

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Advertisements

Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters Artem Vorobyev, Hideyuki Ujiie, Andreas Recke,

Blockade of PDGF Receptors by Crenolanib Has Therapeutic Effect in Patient Fibroblasts and in Preclinical Models of Systemic Sclerosis Katsunari Makino,

IFN-γ Primes Keratinocytes for HSV-1–Induced Inflammasome Activation

Re-Epithelialization of Pathological Cutaneous Wounds Is Improved by Local Mineralocorticoid Receptor Antagonism Van Tuan Nguyen, Nicolette Farman, Eve.

Volume 88, Issue 6, Pages (December 2015)

TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid Yale Liu, Lingling Peng, Liang Li, Chengfei Liu, Xiao Hu, Shengxiang Xiao,

Critical Role for Skin-Derived Migratory DCs and Langerhans Cells in TFH and GC Responses after Intradermal Immunization Clément Levin, Olivia Bonduelle,

The Leukotriene B4 and its Receptor BLT1 Act as Critical Drivers of Neutrophil Recruitment in Murine Bullous Pemphigoid-Like Epidermolysis Bullosa Acquisita

IL-21 May Promote Granzyme B-Dependent NK/Plasmacytoid Dendritic Cell Functional Interaction in Cutaneous Lupus Erythematosus Valentina Salvi, William.

Priti Agarwal, Mehdi Rashighi, Kingsley I. Essien, Jillian M

Activation of TGF-β1 by AQP3-Mediated H2O2 Transport into Fibroblasts of a Bleomycin-Induced Mouse Model of Scleroderma Jingying Luo, Xin Liu, Jie Liu,

Dual Role of Act1 in Keratinocyte Differentiation and Host Defense: TRAF3IP2 Silencing Alters Keratinocyte Differentiation and Inhibits IL-17 Responses

Hiroshi Koga, Andreas Recke, Gestur Vidarsson, Hendri H. Pas, Marcel F

Increasing the Complement of Therapeutic Options in Bullous Pemphigoid

The Leukotriene B4 and its Receptor BLT1 Act as Critical Drivers of Neutrophil Recruitment in Murine Bullous Pemphigoid-Like Epidermolysis Bullosa Acquisita

Bullous Pemphigoid Sera that Contain Antibodies to BPAg2 also Contain Antibodies to LABD97 that Recognize Epitopes Distal to the NC16A Domain Conleth.

Heat Increases the Editing Efficiency of Human Papillomavirus E2 Gene by Inducing Upregulation of APOBEC3A and 3G Yang Yang, Hexiao Wang, Xinrui Zhang,

The disturbance of TH17-Treg cell balance in adenomyosis

Myeloid-Derived Suppressor Cells in Psoriasis Are an Expanded Population Exhibiting Diverse T-Cell–Suppressor Mechanisms Lauren Y. Cao, Jin-Sung Chung,

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

Tumor necrosis factor-α production in whole blood after cardiopulmonary bypass: Downregulation caused by circulating cytokine-inhibitory activities J.

Spread of Psoriasiform Inflammation to Remote Tissues Is Restricted by the Atypical Chemokine Receptor ACKR2 Kave Shams, Gillian J. Wilson, Mark Singh,

Volume 20, Issue 12, Pages (September 2017)

Dimethylfumarate Impairs Neutrophil Functions

Refractory oral ulcers with multiple immunoglobulin G/immunoglobulin A autoantibodies without skin lesions Teruki Dainichi, MD, Bungo Ohyama, MD, Norito.

Effects of Intravenous Immunoglobulins on Mice with Experimental Epidermolysis Bullosa Acquisita Misa Hirose, Benjamin Tiburzy, Norito Ishii, Elena Pipi,

C-Terminal Processing of Collagen XVII Induces Neoepitopes for Linear IgA Dermatosis Autoantibodies Ellen Toyonaga, Wataru Nishie, Kentaro Izumi, Ken.

Laser Irradiation of Organic Tattoo Pigments Releases Carcinogens with 3,3′- Dichlorobenzidine Inducing DNA Strand Breaks in Human Skin Cells Henrik Hering,

R. Sommaggio, M. Pérez-Cruz, J.L. Brokaw, R. Máñez, C. Costa

Toll-Like Receptor-Mediated Upregulation of CXCL16 in Psoriasis Orchestrates Neutrophil Activation Sabine Steffen, Susanne Abraham, Maik Herbig, Franziska.

Histamine Contributes to Tissue Remodeling via Periostin Expression

Methylprednisolone Blocks Autoantibody-Induced Tissue Damage in Experimental Models of Bullous Pemphigoid and Epidermolysis Bullosa Acquisita through.

Activation of TRPV3 Inhibits Lipogenesis and Stimulates Production of Inflammatory Mediators in Human Sebocytes—A Putative Contributor to Dry Skin Dermatoses

Association of Serum B-Cell Activating Factor Level and Proportion of Memory and Transitional B Cells with Clinical Response after Rituximab Treatment.

Ketoconazole Suppresses Interleukin-4 plus Anti-CD40-Induced IgE Class Switching in Surface IgE Negative B Cells from Patients with Atopic Dermatitis

Pivotal Role of Lesional and Perilesional T/B Lymphocytes in Pemphigus Pathogenesis Huijie Yuan, Shengru Zhou, Zhicui Liu, Weiting Cong, Xiaochun Fei,

The Antimicrobial Protein Psoriasin (S100A7) Is Upregulated in Atopic Dermatitis and after Experimental Skin Barrier Disruption Regine Gläser, Ulf Meyer-Hoffert,

CpG Oligodeoxynucleotides Prevent the Development of Scleroderma-Like Syndrome in Tight-Skin Mice by Stimulating a Th1 Immune Response Yan Shen, Motohide.

Symptomatic Improvement in Human Papillomavirus-Induced Epithelial Neoplasia by Specific Targeting of the CXCR4 Chemokine Receptor Floriane Meuris, Françoise.

Prevalence and Age Distribution of Pemphigus and Pemphigoid Diseases in Germany Franziska Hübner, Andreas Recke, Detlef Zillikens, Roland Linder, Enno.

Function Blocking Autoantibodies Against Matrix Metalloproteinase-1 in Patients with Systemic Sclerosis Shinichi Sato, Ikuko Hayakawa, Minoru Hasegawa,

Dan F. Spandau, Davina A. Lewis, Ally-Khan Somani, Jeffrey B. Travers

Modes of Action of Intravenous Immunoglobulin in Bullous Pemphigoid

Francois le Pelletier, Anne Janin Journal of Investigative Dermatology

J. Claire Hoving, Natalie E. Nieuwenhuizen, Georgia Schäfer, Arieh A

Autoantibodies to Bullous Pemphigoid Antigen 180 Induce Dermal–Epidermal Separation in Cryosections of Human Skin Cassian Sitaru, Enno Schmidt, Steffen.

Detection of IgG Autoantibodies in the Sera of Patients with Bullous and Gestational Pemphigoid: ELISA Studies Utilizing a Baculovirus-Encoded Form of.

Bullous Pemphigoid, Neurodegenerative Disease, and Hippocampal BP180 Expression: A Retrospective Postmortem Neuropathologic Study Benjamin J. Barrick,

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

Identification of a Potential Effector Function for IgE Autoantibodies in the Organ- Specific Autoimmune Disease Bullous Pemphigoid Otobia G. Dimson,

Topical Acyclothymidine Dinucleosides (aTds) Promote Non-UV-Mediated Endogenous Defense Mechanisms in Guinea Pig Skin Jaime S. Nugent, Robert Vince,

Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature Jessica S.

TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid Yale Liu, Lingling Peng, Liang Li, Chengfei Liu, Xiao Hu, Shengxiang Xiao,

Autophagy Has a Significant Role in Determining Skin Color by Regulating Melanosome Degradation in Keratinocytes Daiki Murase, Akira Hachiya, Kei Takano,

Increased Expression of Endothelial Cell Adhesion Molecules Due to Mediator Release from Human Foreskin Mast Cells Stimulated by Autoantibodies in Chronic.

Masaki Nagai, Minoru Hasegawa, Kazuhiko Takehara, Shinichi Sato

Interferon-γ, a Strong Suppressor of Cell Proliferation, Induces Upregulation of Keratin K6, One of the Inflammatory- and Proliferation-Associated Keratins

Decreased Phospholipase D (PLD) Activity in Ceramide-Induced Apoptosis of Human Keratinocyte Cell Line HaCaT Yoshihiko Iwasaki-Bessho, Yoshiko Banno,

FcγRIIA and FcγRIIIB Are Required for Autoantibody-Induced Tissue Damage in Experimental Human Models of Bullous Pemphigoid Xinhua Yu, Karoline Holdorf,

Dawn Cooper, Jonathan Hales, Richard Camp

Autoantibody in Mucous Membrane Pemphigoid Binds to an Intracellular Epitope on Human β4 Integrin and Causes Basement Membrane Zone Separation in Oral.

Autoantibodies to BP180 Associated with Bullous Pemphigoid Release Interleukin-6 and Interleukin-8 from Cultured Human Keratinocytes Enno Schmidt, Stanislaus.

Increased Levels of the Bullous Pemphigoid BP180 Autoantibody Are Associated with More Severe Dementia in Alzheimer’s Disease Nina Kokkonen, Sanna-Kaisa.

Janet A. Fairley, Chang Ling Fu, George J. Giudice

Detection of Laminin 5-Specific Auto-antibodies in Mucous Membrane and Bullous Pemphigoid Sera by ELISA Vassiliki Bekou, Sybille Thoma-Uszynski, Olaf.

Severity and Phenotype of Bullous Pemphigoid Relate to Autoantibody Profile Against the NH2- and COOH-Terminal Regions of the BP180 Ectodomain Silke.

Generation of Antibodies of Distinct Subclasses and Specificity Is Linked to H2s in an Active Mouse Model of Epidermolysis Bullosa Acquisita Ralf J.

Zhuxiang Nie, Takashi Hashimoto Journal of Investigative Dermatology

Recombinant Soluble CD32 Suppresses Disease Progression in Experimental Epidermolysis Bullosa Acquisita Hiroaki Iwata, Elena Pipi, Nicole Möckel, Peter.

Presentation transcript:

The Anti-C1s Antibody TNT003 Prevents Complement Activation in the Skin Induced by Bullous Pemphigoid Autoantibodies Anika Kasprick, Maike M. Holtsche, Eileen L. Rose, Sami Hussain, Enno Schmidt, Frank Petersen, Sandip Panicker, Ralf J. Ludwig Journal of Investigative Dermatology Volume 138, Issue 2, Pages 458-461 (February 2018) DOI: 10.1016/j.jid.2017.08.030 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Anaphylatoxin levels in plasma of patients with BP. In an exploratory study, levels of anaphylatoxins C3a, C4a, and C5a were determined in plasma samples of (a) patients with BP before initial treatment and sex- and age-matched controls (n = 16/group). Data are shown as individual symbols with mean ± standard deviation, and statistically significant differences between groups are indicated (unpaired t-test). (b) Data of nine patients before and 3 months after initial treatment are shown, and statistically significant differences between time points are indicated (paired t-test, one-tailed). (c) Anaphylatoxin levels of 16 patients with BP before initial treatment were correlated to serum titers of anti-BP180-specific autoantibodies from the same time point. Each data point represents one patient and line indicates linear regression (r, Spearman correlation coefficient). BP, bullous pemphigoid. Journal of Investigative Dermatology 2018 138, 458-461DOI: (10.1016/j.jid.2017.08.030) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Inhibition of anaphylatoxin formation and complement C3 deposition by TNT003. Healthy human foreskin was incubated with serum from patients with bullous pemphigoid (BP sera) or healthy humans (NHS) followed by human plasma in the absence or presence of an isotype (TNT001) or anti-C1s (TNT003) antibody, respectively. Treatment with EDTA served as positive control for complement inhibition. (a) Incubated tissue was stained for complement C3c and its deposition semiquantified at a fluorescence microscope (BP, n = 18; NHS, n = 7). Representative photographs of each condition are shown (scale bar = 50 μm). (b) C3a (n = 4), C4a (n = 5), and C5a (n = 5) were measured in the supernatants of the complement activation assay and data were normalized to untreated samples (---). Data are shown as mean ± SD and statistically significant differences between groups are indicated (one-way ANOVA and multiple comparison with Bonferroni’s method; ns, not significant; **P < 0.01; ***P < 0.001). ANOVA, analysis of variance; PBS, phosphate buffered saline; SD, standard deviation. Journal of Investigative Dermatology 2018 138, 458-461DOI: (10.1016/j.jid.2017.08.030) Copyright © 2017 The Authors Terms and Conditions