Administration of pIL2 and pgDE7 induces higher activation of E7-specific Tem CD8+ T cells. Administration of pIL2 and pgDE7 induces higher activation.

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Administration of pIL2 and pgDE7 induces higher activation of E7-specific Tem CD8+ T cells. Administration of pIL2 and pgDE7 induces higher activation of E7-specific Tem CD8+ T cells. Groups of 5 mice were challenged with 7.5 × 104 TC-1 cells and immunized 3 days later with one dose of pgDE7, pIL2, or both plasmids combined (50 μg of each plasmid). Spleens were harvested 14 days after vaccination, or blood was collected weekly after challenge. Splenocytes and blood cells were stimulated in vitro overnight with a peptide from HPV-16 E7 corresponding to the Kb MHC class I–restricted epitope. A, percentage of CD8+ T cells producing IFNγ or simultaneously IFNγ and CD107a from splenocytes. B, percentage of CD8+ T cells producing IFNγ after stimulation with serial dilutions of E7 peptide from splenocytes. ns, not significant. C, percentage of CD8+ T cells with a Tcm or Tem phenotype from splenocytes. Numbers on top, Tem/Tcm ratio. D, percentages of IFNγ-producing Tcm or Tem cells; Tcm, central memory CD8+ T cells (CD8+CD44highCD62L+); Tem, effector memory CD8+ T cells (CD8+CD44highCD62L). E, percentage of IFNγ-producing CD8+ T cells over time from blood samples. The data are from one representative experiment of three performed. The data are expressed as the mean ± SD. *, P < 0.05; **, P < 0.005; ***, P < 0.001; ns, not significant. Mariana O. Diniz et al. Mol Cancer Ther 2016;15:1920-1930 ©2016 by American Association for Cancer Research