Affinity purification of latex antigens

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Presentation transcript:

Affinity purification of latex antigens Jay E. Slater, MD, Diane E. Trybul, BS Journal of Allergy and Clinical Immunology Volume 93, Issue 3, Pages 644-649 (March 1994) DOI: 10.1016/S0091-6749(94)70076-1 Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 1 Fifteen percent SDS-PAGE of (lane a) nonreduced NAL and C-Ag, (lane b) reduced NAL and C-Ag. Molecular weight markers are indicated on the right and left. Journal of Allergy and Clinical Immunology 1994 93, 644-649DOI: (10.1016/S0091-6749(94)70076-1) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 2 AL-RAST inhibition, with pooled sera from (A) children with spina bifida and latex allergy or (B) health care workers with latex allergy. O, NAL; X, C-Ag. Each point is the average of three experiments ± standard deviation. Journal of Allergy and Clinical Immunology 1994 93, 644-649DOI: (10.1016/S0091-6749(94)70076-1) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 2 AL-RAST inhibition, with pooled sera from (A) children with spina bifida and latex allergy or (B) health care workers with latex allergy. O, NAL; X, C-Ag. Each point is the average of three experiments ± standard deviation. Journal of Allergy and Clinical Immunology 1994 93, 644-649DOI: (10.1016/S0091-6749(94)70076-1) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 3 Immunoblot with antibody CRI-C of (lane a) nonreduced NAL, (lane b) reduced NAL, (lane c) nonreduced AL, and (lane d) reduced AL. Molecular weight markers are indicated on the left. Journal of Allergy and Clinical Immunology 1994 93, 644-649DOI: (10.1016/S0091-6749(94)70076-1) Copyright © 1994 Mosby, Inc. Terms and Conditions

FIG. 4 IgE immunoblot inhibition of separated reduced NAL. Lanes a to c, Pooled spina bifida sera; lanes d to f, pooled health care worker sera. Antigen added: lanes c and f, none; lanes b and e, NAL; and lanes a and d, C-Ag. Journal of Allergy and Clinical Immunology 1994 93, 644-649DOI: (10.1016/S0091-6749(94)70076-1) Copyright © 1994 Mosby, Inc. Terms and Conditions