Molecular characterization of a cross-reactive Juniperus oxycedrus pollen allergen, Jun o 2: A novel calcium-binding allergen Raffaella Tinghino, BSc,

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Molecular characterization of a cross-reactive Juniperus oxycedrus pollen allergen, Jun o 2: A novel calcium-binding allergen Raffaella Tinghino, BSc, Bianca Barletta, BSc, Sabrina Palumbo, BSc, Claudia Afferni, BSc, Patrizia Iacovacci, BSc, Adriano Mari, MD, Gabriella Di Felice, BSc, Carlo Pini, BSc Journal of Allergy and Clinical Immunology Volume 101, Issue 6, Pages 772-777 (June 1998) DOI: 10.1016/S0091-6749(98)70306-9 Copyright © 1998 Mosby, Inc. Terms and Conditions

Figure 1 Results of comparative analysis between rJun o 2 and CALMPYUSP, calmodulin from sea squirt (EMBL accession no. P11121). Residues forming calcium-binding motifs are indicated with # . Bars represent gaps that have been introduced just to align similar portions (calcium-binding sites) and to ensure maximum homology. Identical and similar residues between two sequences are indicated with vertical bar. Journal of Allergy and Clinical Immunology 1998 101, 772-777DOI: (10.1016/S0091-6749(98)70306-9) Copyright © 1998 Mosby, Inc. Terms and Conditions

Figure 2 Effect of protein-bound Ca 2+ on IgE-binding capacity of rJun o 2. Plaque-lift nitrocellulose sectors containing rJun o 2 were incubated with serum IgE from individual allergic to Cupressaceae. Filters were incubated either with serum only (A) or with serum in presence of EGTA (B). After development of both filters with 125I-anti-human IgE, filter B was probed with newly added human serum in absence of EGTA and further developed with 125I-anti-human IgE (C). Journal of Allergy and Clinical Immunology 1998 101, 772-777DOI: (10.1016/S0091-6749(98)70306-9) Copyright © 1998 Mosby, Inc. Terms and Conditions

Figure 3 A, SDS-PAGE analysis of JoE (lane 1), noninduced cell lysate (lane 2), isopropyl β-thiogalactoside–induced cell lysate (lane 3), and affinity-purified rJun o 2 (lane 4). B, Immunoblotting analysis of transferred JoE (lane 1), crude noninduced cell lysate (lane 2), affinity-purified rJun o 2 (lane 3) developed with pooled human sera from subjects allergic to Cupressaceae, and immunoblotting analysis of transferred affinity-purified rJun o 2 developed with human serum from a nonallergic individual (lane 4). C, Immunoblotting inhibition pattern of rJun o 2 after incubation of human serum from one subject allergic to Cupressaceae with 25 (lane 1), 5 (lane 2), and 1 (lane 3) μg protein/ml of JoE. Lane 4, No inhibitor added. MW, Molecular weight. Journal of Allergy and Clinical Immunology 1998 101, 772-777DOI: (10.1016/S0091-6749(98)70306-9) Copyright © 1998 Mosby, Inc. Terms and Conditions

Figure 4 Immunoblotting inhibition pattern of IgE binding to rJun o 2 inhibited with pollen extracts from Cupressaceae (A) or from taxonomically unrelated families (B). Amounts of inhibitors are 25, 5, and 1 μg protein/ml of JaE (A, lanes 1 to 3); 50, 25, and 5 μg protein/ml of CaE (A, lanes 4 to 6) and CsE (A, lanes 7 to 9); 5, 1, and 0.25 μg protein/ml of PjE (B, lanes 1 to 3); 50, 25, and 5 μg protein/ml of OeE (B, lanes 4 to 6); and 50, 25, and 5 μg protein/ml of LpE ( B, lanes 7 to 9). Lane 10 (A), No inhibitor added. Journal of Allergy and Clinical Immunology 1998 101, 772-777DOI: (10.1016/S0091-6749(98)70306-9) Copyright © 1998 Mosby, Inc. Terms and Conditions