Stat3C/C mice are more prone to Th17 differentiation. A

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Stat3C/C mice are more prone to Th17 differentiation. A Stat3C/C mice are more prone to Th17 differentiation.A.Cytofluorimetric analysis of IL‐17 producing CD4+ T cells in the spleen of Stat3WT/WT (white bars, n = 6) and Stat3C/C (black bars, n = 6) mice. Stat3C/C mice are more prone to Th17 differentiation.A.Cytofluorimetric analysis of IL‐17 producing CD4+ T cells in the spleen of Stat3WT/WT (white bars, n = 6) and Stat3C/C (black bars, n = 6) mice. Data are shown as mean ± SEM. *p = 0.015.B.In vitro differentiation of Th17 cells. Naïve CD4+ cells were purified from spleens of Stat3WT/WT (white bars, n = 6) and Stat3C/C (black bars, n = 10) mice and treated or not with TGF‐β, IL‐6 and anti‐IFN‐γ, followed by cytofluorimetric analysis for CD4 and IL‐17. Data are shown as mean ± SEM. **p = 0.001.C.In vitro differentiation of Th17 cells without IL‐6. Naïve CD4+ cells were purified from spleens of Stat3WT/WT (white bars, n = 6) and Stat3C/C (black bars, n = 6) mice, treated or not with TGF‐β and anti‐IFN‐γ and analysed as above. Note the different scales between (B) and (C). Data are shown as mean ± SEM. **p = 0.006 (Stat3WT/WTversus Stat3C/C cells); *p = 0.025 (untreated versus treated Stat3C/C cells).D.Taqman RT‐PCR analysis on total RNA from the spleen of Stat3WT/WT (white bars, n = 7) and Stat3C/C (black bars, n = 7) mice. Data are shown as mean ± SEM. *p = 0.018 (RORγt); **p = 0.0057 (IL‐23).E.IL‐17 (red) and CD4 (green) IF staining of hearts from Stat3WT/WT (WT) and Stat3C/C (C) mice at the indicated weeks of age. Representative samples of three mice per group are shown. Scale bar: 50 µm.F.Kaplan‐Meier survival curves of Stat3C/C mice treated with anti‐IL‐17 neutralizing mAbs (red line, n = 7) or with total IgG as controls (black line, n = 4).G.IL‐17 (red) and CD4 (green) IF staining of hearts from anti IL‐17‐treated and control mice as above. Representative samples from survived (n = 2) or diseased (n = 5) mice are shown. Scale bar: 50 µm.H.Kaplan‐Meier survival curves of Stat3C/C mice treated with anti‐CD4 neutralizing mAbs (red line, n = 13) or with total IgGs as controls (black line, n = 10). *p = 0.026.I.The hearts of mice from (H) were analysed as indicated. IgG, control mice; for the aCD4‐treated mice, samples from survived mice are shown on the left, and those from dead mice on the right. Scale bar: 50 µm. Annalisa Camporeale et al. EMBO Mol Med. 2013;5:572-590 © as stated in the article, figure or figure legend