Measurement of matrilin-3 levels in human serum and synovial fluid using a competitive enzyme-linked immunosorbent assay J.-B. Vincourt, P. Gillet, A.-C.

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Measurement of matrilin-3 levels in human serum and synovial fluid using a competitive enzyme-linked immunosorbent assay J.-B. Vincourt, P. Gillet, A.-C. Rat, F. Guillemin, P. Netter, D. Mainard, J. Magdalou Osteoarthritis and Cartilage Volume 20, Issue 7, Pages 783-786 (July 2012) DOI: 10.1016/j.joca.2012.03.017 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Saturation curve and specifications of the assay. (A) Classically observed dose-response of the assay represented on a logarithmic concentration scale. The equation of the regression curve together with the correlation coefficient (r2) are annotated. The plot shows average measures and bars indicate SD. (B) summary of the specifications of the assay. Osteoarthritis and Cartilage 2012 20, 783-786DOI: (10.1016/j.joca.2012.03.017) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Specificity of the antibody and adaptation of the assay to SFs and sera. (A) Proteomic investigation of the specificity of the antibody used in the assay. SFs found by ELISA to contain high versus low MATN3 amounts were subjected to immunoprecpitation using the same antibody and the immunoprecipitates were analyzed by 2D-electrophoresis coupled mass spectrometry. Spots of interest are indicated by arrows. (B) Comparison of SF and serum contents in Matrilin-3 from 10 patients. Segments link measures of both compartments for each individual patient. SD were not indicated to allow visualization of details. The indicated p value is as calculated by the student t test. Osteoarthritis and Cartilage 2012 20, 783-786DOI: (10.1016/j.joca.2012.03.017) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Osteoarthritis and Cartilage 2012 20, 783-786DOI: (10. 1016/j. joca Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions