Expression profiles of GAS virulence factors during planktonic and biofilm growth. Expression profiles of GAS virulence factors during planktonic and biofilm growth. (A) z-scored expression values for 54 characterized and putative GAS virulence factor genes. The putative phage hyaluronidase genes are denoted with the symbol “hyl” followed by the predicted molecular mass. (B) z-scored expression values for the 14 of the 54 GAS virulence factors with corresponding proteomic data in the cellular fraction. (C) z-scored expression values for the 12 of the 54 GAS virulence factors with corresponding proteomic data in the cell wall fraction. Jeffrey A. Freiberg et al. mSystems 2016; doi:10.1128/mSystems.00149-16