This Month in Gastroenterology Laura Harrell, Eugene B. Chang  Gastroenterology  Volume 129, Issue 5, Pages 1367-1369 (November 2005) DOI: 10.1053/j.gastro.2005.09.048 Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 1 Enhanced Smad7 protein but not RNA expression in IBD. (A) Abundance of Smad7 RNA transcripts in 12 control, 11 CD, and 5 UC mucosal samples by real-time PCR. Each point represents the Smad7/β2 microglobulin RNA ratio in mucosal biopsies taken from a single subject. Values are expressed in arbitrary units of fluorescence × 10(-7). Horizontal bars indicate the median value. (B) Representative expression of Smad7 (upper blot) and β-actin (lower blot) protein in mucosal samples taken from 2 controls, 2 patients with Crohn’s disease (CD), and 2 with ulcerative colitis (UC). The example is representative of 3 separate experiments analyzing in total mucosal samples from 12 controls, 11 CD, and 5 UC patients. Right inset shows the content of Smad7/β2 microglobulin RNA in paired mucosal biopsies taken from the same 2 controls, 2 patients with CD, and B 2 with UC in which the expression of Smad7 protein. Values are expressed in arbitrary units of fluorescence × 10(−7). Gastroenterology 2005 129, 1367-1369DOI: (10.1053/j.gastro.2005.09.048) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 2 Immunohistochemical analysis of the expression of p300. In all IBD samples (representative image of CD shown in A), p300 protein was clearly seen in cells in the lamina propria, with strong nuclear staining. In control samples, a very weak staining was seen (C). Control IgG gave no staining on both CD (B) and control (D) samples; (immunoperoxidase, original magnification 400×). Gastroenterology 2005 129, 1367-1369DOI: (10.1053/j.gastro.2005.09.048) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 3 Kaplan–Meier analysis of survival without liver transplantation in UDCA and placebo-treated patients. Filled circles = patients who died or had a liver transplantation. Gastroenterology 2005 129, 1367-1369DOI: (10.1053/j.gastro.2005.09.048) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 4 Changes from start in serum ALP, ALT, and bilirubin. Mean with SD. Gastroenterology 2005 129, 1367-1369DOI: (10.1053/j.gastro.2005.09.048) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 5 Representative proximal colons from 70-week-old male mice. There is a marked histologic difference between (A) C3Bir.Il10-/- and (B) B6.Il10-/- mice. The C3Bir.Il10-/- mice develop ulceration (center, A) associated with marked acute inflammation and exudation, as well as epidermal hyperplasia with inflammation within the lamina propria in adjacent regions. In contrast, B6.Il10-/- mice are frequently free of lesions in this region. The sample from the CB-R1 stock (C), in which the genetic interval containing Cdcs1 from C3Bir was replaced with the B6 resistant allele, has no disease phenotype. (D) In contrast, the sample from the reciprocal congenic BC-R1 stock, in which the B6-resistant Cdcs1 allele was replaced with the C3Bir colitis susceptibility allele, has severe (D) ulcerative and hyperplastic colon lesions. Gastroenterology 2005 129, 1367-1369DOI: (10.1053/j.gastro.2005.09.048) Copyright © 2005 American Gastroenterological Association Terms and Conditions