Olivier Donnez, M. D. , Michelle Soares, M. D. , Sylvie Defrère, Ph. D

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Presentation transcript:

Nerve fiber density in deep nodular endometriotic lesions induced in a baboon experimental model Olivier Donnez, M.D., Michelle Soares, M.D., Sylvie Defrère, Ph.D., Jean-Paul Dehoux, D.V.M., Ph.D., Anne van Langendonckt, Ph.D., Jacques Donnez, M.D., Ph.D., Marie-Madeleine Dolmans, M.D., Ph.D., Sébastien Colette, Ph.D. Fertility and Sterility Volume 100, Issue 4, Pages 1144-1150.e2 (October 2013) DOI: 10.1016/j.fertnstert.2013.06.014 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Microscopic appearance of an invasive lesion through the sigmoid muscularis, with (A) hematoxylin-eosin staining and (B) cytokeratin-22 immunostaining evidencing glands. Invasive lesions were characterized by the presence of endometriotic glands and stroma (arrows) inside surrounding organs. Scale bars = 200.0 μm. Fertility and Sterility 2013 100, 1144-1150.e2DOI: (10.1016/j.fertnstert.2013.06.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Protein gene product (PGP) 9.5 and nerve growth factor (NGF) immunostaining in baboon uteri and endometriotic lesions. PGP9.5 and NGF were immunostained in (A and B, respectively) eutopic baboon uteri, and (C and D, respectively) noninvasive and (E and F, respectively) invasive lesions. E and F represent glands inside surrounding organs. Glands could be followed along serial sections from the initial lesion. Scale bars = 100.0 μm (A, C), 50 μm (E), and 200.0 μm (B, D, F). Fertility and Sterility 2013 100, 1144-1150.e2DOI: (10.1016/j.fertnstert.2013.06.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Nerve fiber densities. Nerve fiber density (NFD) was measured in (A) eutopic baboon endometrium and lesions, as well as (B) their direct environment (<1.5 mm). *P < .05; ***P < .001. Fertility and Sterility 2013 100, 1144-1150.e2DOI: (10.1016/j.fertnstert.2013.06.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Nerve growth factor (NGF) expression analysis. NGF expression was scored for baboon (A) glands and (B) stroma. *P < .05. Fertility and Sterility 2013 100, 1144-1150.e2DOI: (10.1016/j.fertnstert.2013.06.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Photomicrographs illustrating negative control samples: protein gene product (PGP) 9.5 immunostaining of an endometriotic lesion processed (A) with nonspecific IgG instead of the primary antibody and (B) with anti-PGP9.5 antibody; nerve fiber density (NFD) immunostaining of an endometriotic lesion (C) with nonspecific IgG instead of the primary antibody and (D) with anti–nerve growth factor (NGF) antibody. Fertility and Sterility 2013 100, 1144-1150.e2DOI: (10.1016/j.fertnstert.2013.06.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions