* * Supplementary figure S4 A B C D

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* * Supplementary figure S4 A B C D Src-1 siRNA Scram siRNA E6/E7-HFK Inset 100µm Total Src 60 kDa β-actin 44 kDa Src-pY416 siRNA Scram Slug mol-1 Slug mol-2 Src-pY416 Total Src Slug 30 kDa Vimentin 57 kDa β-actin 44 kDa 60 kDa GFP Src-531 E-cadherin 120 KDa pBabe-HFK * 100µm Inset C D Figure S4: (A) Protein expressions of Src-pY416, total Src and β-actin in Scram siRNA (control), Slug siRNA mol-1 and mol-2 transfected E6/E7-HFK; (B) Phase contrast images indicating EMT (red arrows) and normal (green asterisk) morphology in Scram and Src mol-1 siRNA transfected E6/E7-HFK. (C-D) Protein expressions of total Src, Src-pY416, Slug, vimentin, E-cadherin and β-actin in lysates from non-invasive HFK expressing either GFP or constitutively active Src (Src-531). Phase contrast images indicating EMT (red arrows) and normal (green asterisk) morphology in these cells. Scale bars represented as 100μm. N=3 independent experiments.