Erratum Journal of Investigative Dermatology

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Erratum Journal of Investigative Dermatology Journal of Investigative Dermatology Volume 138, Issue 2, Pages 472-473 (February 2018) DOI: 10.1016/j.jid.2017.12.014 Copyright © 2017 The Authors Terms and Conditions

Figure 1 IL-4 attenuates the expression levels of Krt1, Krt10, Dsg1a, and Dsc1 in differentiating primary mouse keratinocytes. The keratinocytes were differentiated for 9 days. IL-4 was added for the last 2 days of the culture. A real-time PCR analysis of (a) Krt1, Krt5, Krt10, Krt14, (b) Dsg1a, Dsg3, Dsc1, and Dsc3 with or without IL-4 stimulation. The data were normalized to the Ppia mRNA expression using the comparative CT method. The relative quantity (RQ) of genes is depicted as the mRNA expression in the differentiating keratinocytes without any cytokines (n = 7). Asterisk, statistically significant (a matched-pair analysis). A western blotting analysis of (c) keratin 1 (KRT1), KRT5, KRT10, KRT14, and actin (n = 3) and (e) desmoglein 1 (DSG1) and desmocollin 1 (DSC1; n = 3). The amount of soluble fraction of each lysate in the right lane is one-third of that presented in the left lane. (d, f) Densitometry plots of c and e. RD, relative density; asterisk, statistically significant (a matched-pair analysis). Journal of Investigative Dermatology 2018 138, 472-473DOI: (10.1016/j.jid.2017.12.014) Copyright © 2017 The Authors Terms and Conditions

Figure 5 IL-4 or IL-13 attenuates the mRNA expression levels of KRT1, KRT10, DSG1, and DSC1 and increases the susceptibility of human keratinocyte HaCaT cell sheets to mechanical stress. The keratinocytes were differentiated for 5 days. IL-4 or IL-13 was added for the last 2 days of the culture. A real-time PCR analysis of (a) KRT1, KRT5, KRT10, KRT14, (b) DSG1, DSG3, DSC1, and DSC3 with or without cytokine stimulation. The data were normalized to the TBP mRNA expression using the comparative CT method. The relative quantities (RQs) of the genes (n = 4). Asterisks in a and b, statistically significant (one-way analysis of variance (ANOVA) with Dunnett’s post hoc test). (c) A western blotting analysis of keratin 1 (KRT1), KRT10, desmoglein 1 (DSG1), desmocollin 1 (DSC1), and actin (n = 3). Densitometry plots are depicted. RD, relative density; asterisk, statistically significant (one-way ANOVA with Dunnett’s post hoc test). (d) The degree of keratinocyte clustering in the presence or absence of cytokines was depicted (upper) and quantified (bottom) using a dispase-based assay (n = 4). Asterisk, statistically significant (a matched-pair analysis). Journal of Investigative Dermatology 2018 138, 472-473DOI: (10.1016/j.jid.2017.12.014) Copyright © 2017 The Authors Terms and Conditions