Modified Real-Time PCR for Detecting, Differentiating, and Quantifying Ureaplasma urealyticum and Ureaplasma parvum Ellen Vancutsem, Oriane Soetens,

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Presentation transcript:

Modified Real-Time PCR for Detecting, Differentiating, and Quantifying Ureaplasma urealyticum and Ureaplasma parvum Ellen Vancutsem, Oriane Soetens, Maria Breugelmans, Walter Foulon, Anne Naessens The Journal of Molecular Diagnostics Volume 13, Issue 2, Pages 206-212 (March 2011) DOI: 10.1016/j.jmoldx.2010.10.007 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Standard curve (in triplicate) from plasmids containing the U. parvum serotype 3 DNA fragment. Correlation coefficient, 0.996; slope, −3.305; intercept, 43.318; PCR efficiency, 100.7%. The Journal of Molecular Diagnostics 2011 13, 206-212DOI: (10.1016/j.jmoldx.2010.10.007) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Standard curve in triplicate from plasmids containing the U. urealyticum serotype 10 DNA fragment. Correlation coefficient, 0.995; slope, −3.108; intercept, 43.601; PCR efficiency, 109.8%. The Journal of Molecular Diagnostics 2011 13, 206-212DOI: (10.1016/j.jmoldx.2010.10.007) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Comparison of the semiquantitative culture results (xaxis) (133 culture-positive samples) and the quantitative PCR results (y axis). The qPCR results are split into two groups: quantification of U. urealyticum and U. parvum. The Journal of Molecular Diagnostics 2011 13, 206-212DOI: (10.1016/j.jmoldx.2010.10.007) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions