Fertility and Sterility Light and electron microscopic evaluation of antileukotriene therapy for experimental rat endometriosis  Tomomi Ihara, M.D., Ph.D., Ichiro Uchiide, M.D., Ph.D., Masao Sugamata, M.D., Ph.D.  Fertility and Sterility  Volume 81, Pages 819-823 (March 2004) DOI: 10.1016/j.fertnstert.2003.08.029

FIGURE 1 Histopathologic comparison of nontreated and LT-R antagonist-treated rat endometriosis. (a–b) Nontreated rat endometriosis at 4 days after, and enlargement of rectangular area. (Stain: H&E. Magnification: a: ×10 objective, ×10 ocular; b: ×4 objective, ×10 ocular.) (c–d) LT-R antagonist-treated rat endometriosis at 4 days after, and enlargement of rectangular area. (Stain: H&E. Magnification: c: ×10 objective, ×10 ocular; d: ×4 objective, ×10 ocular.) am = abdominal muscle; pc = peritoneal cavity; asterisk = transplanted uterine; arrowhead = basement membrane; at both ends of broad arrow = proliferative lesion area. Ihara. LT-R antagonist-treated endometriosis.Fertil Steril 2004. Fertility and Sterility 2004 81, 819-823DOI: (10.1016/j.fertnstert.2003.08.029)

FIGURE 2 The comparison of mast cell count per light microscopic field for nontreated and LT-R antagonist-treated rat endometriosis. (Magnification: ×40 objective, ×10 ocular.) The bar graphs indicate the mean mast cell count, and error bars indicate ±1 SD. Statistical analysis was performed using the Mann-Whitney U test. ▩ = undegranulated mast cells count. ■ = degranulated mast cells count. Ihara. LT-R antagonist-treated endometriosis.Fertil Steril 2004. Fertility and Sterility 2004 81, 819-823DOI: (10.1016/j.fertnstert.2003.08.029)

FIGURE 3 Light and electron microscopic observations of nontreated (a–d) and LT-R antagonist-treated (e–h) rat endometriosis. (a) Light microscopic observation at 4 days after uterine autotransplantation. (Stain: toluidine blue. Magnification: ×100 objective, ×10 ocular.) Infiltration of mast cells (M) and fibroblasts (F) is observed in the proliferative lesion area of peritoneum autotransplanted from the uterus. (b) Electron microscopic observation at 4 days after uterine autotransplantation. In the peritoneum autotransplanted from the uterus, proliferation of fibroblasts (F) can be seen, and thick collagen fibers (C) have formed around these cells. (Scale bar = 3 μm). (c) Light microscopic observation at 7 days after uterine autotransplantation. (Stain: toluidine blue. Magnification: ×100 objective, ×10 ocular.) Infiltration of mast cells (M) and fibroblasts (F) is observed in the proliferative lesion area of peritoneum autotransplanted from the uterus. (d) Electron microscopic observation at 7 days after uterine autotransplantation. In the peritoneum autotransplanted from the uterus, thick collagen fibers (C) are formed densely around some fibroblasts (F). (Scale bar = 3 μm.) (e) Light microscopic observation at 4 days after uterine autotransplantation. (Stain: toluidine blue. Magnification: ×100 objective, ×10 ocular.) Numerous apoptosis of proliferative fibroblasts (arrow) are observed in peritoneum autotransplanted from the uterus. Some apoptotic fibroblasts have come off the peritoneal tissue. (f) Electron microscopic observation at 4 days after uterine autotransplantation. The peritoneum autotransplanted from the uterus shows apoptosis of proliferative fibroblasts (F), which in turn show nuclear fragmentation (*). Collagen fibers are thin and have disappeared around these apoptotic fibroblasts. Arrow indicates “CSS,” a sign of apoptotic induction. (Scale bar = 5 μm.) (g) Light microscopic observation at 7 days after uterine autotransplantation (Stain: toluidine blue. Magnification: ×100 objective, ×10 ocular.) Some macrophages (m) and phagocytized apoptotic bodies (*) are observed in peritoneum autotransplanted from the uterus. (h) Electron microscopic observation at 7 days after uterine autotransplantation. The peritoneum autotransplanted from the uterus shows macrophage (m) phagocytizes and numerous apoptotic bodies (*). Collagen fibers are thin and have disappeared around the macrophage. (E = eosinophil. Scale bar = 3 μm.) Ihara. LT-R antagonist-treated endometriosis.Fertil Steril 2004. Fertility and Sterility 2004 81, 819-823DOI: (10.1016/j.fertnstert.2003.08.029)