A.W. Palmer, C.G. Wilson, E.J. Baum, M.E. Levenston

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General Information Osteoarthritis and Cartilage

Presentation transcript:

Composition-function relationships during IL-1-induced cartilage degradation and recovery A.W. Palmer, C.G. Wilson, E.J. Baum, M.E. Levenston Osteoarthritis and Cartilage Volume 17, Issue 8, Pages 1029-1039 (August 2009) DOI: 10.1016/j.joca.2009.02.009 Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 sGAG (A) and collagen (B) release to the media over 48h period for control (●) and IL-1-stimulated (○) explants. Data are mean±SEM. * denotes difference (p<0.05) between control and IL-1-stimulated explants. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Residual sGAG content (A), residual collagen content (B), water content (C) and thickness (D) as a function of culture time for control (●) and IL-1-stimulated (○) explants. Data are mean±SEM. * denotes difference (p<0.05) between control and IL-1-stimulated explants. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Mitochondrial activity as measured by conversion of WST-1 (A) and cell viability as indicated by calcein AM (green; live) and ethidium homodimer-1 (red; dead) fluorescence (B) for control (●) and IL-1-stimulated (○) explants. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Compressive and shear properties as a function of culture time for control (●) and IL-1-stimulated (○) explants: (A) Equilibrium unconfined compression modulus; (B) Dynamic unconfined compression modulus at 0.5Hz; (C) Dynamic shear modulus at 1Hz. Data are mean±SEM. * denotes difference (p<0.05) between control and IL-1-stimulated explants. † denotes p=0.06 between control and IL-1-stimulated explants. Ø denotes an insufficient number of IL-1-stimulated explants to perform the statistical analysis. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Dynamic unconfined compression modulus as a function of frequency and culture time for control (A) and IL-1-stimulated (B) explants. Data are mean±SEM. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Dynamic shear modulus as a function of frequency and culture time for control (A) and IL-1-stimulated (B) explants. Data are mean±SEM. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 sGAG (A) and collagen (B) release to the media over 48h period for control (●) and IL-1-stimulated (○) explants. Data are mean±SEM. * denotes difference (p<0.05) between control and IL-1-stimulated explants. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 8 Residual sGAG content (A), residual collagen content (B), water content (C) and thickness (D) as a function of culture time for control (●) and explants recovering from a 4-day exposure to IL-1 (○). Data are mean±SEM. * denotes difference (p<0.05) between control and IL-1-stimulated explants. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 9 Dynamic unconfined compression modulus at 1.0Hz (A) and dynamic shear modulus at 1.0Hz (B) as functions of culture time for control (●) explants and explants following a 4-day transient exposure to IL-1 (○). Data are mean±SEM. * denotes difference (p<0.05) between control and IL-1-stimulated explants. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 10 Physical properties plotted against sGAG concentration (A–C) and collagen concentration (D–F) for control (●) and IL-1-stimulated (○) explants. Note that data are presented on logarithmic scales. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 11 Physical properties plotted against sGAG concentration (A-B) and collagen concentration (C-D) for control (●) and IL-1-stimulated (○) explants. Note that data are presented on logarithmic scales. Osteoarthritis and Cartilage 2009 17, 1029-1039DOI: (10.1016/j.joca.2009.02.009) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions