Lusine Aghajanova, M. D. , Ph. D. , Signe Altmäe, Ph. D

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Presentation transcript:

Stanniocalcin-1 expression in normal human endometrium and dysregulation in endometriosis Lusine Aghajanova, M.D., Ph.D., Signe Altmäe, Ph.D., Sergo Kasvandik, M.Sc., Andres Salumets, Ph.D., Anneli Stavreus-Evers, Ph.D., Linda C. Giudice, M.D., Ph.D. Fertility and Sterility Volume 106, Issue 3, Pages 681-691.e1 (September 2016) DOI: 10.1016/j.fertnstert.2016.05.023 Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Stanniocalcin-1 (STC1) protein level is increased in receptive (LH+8) phase endometrial secretome. Paired data from six fertile women are presented. X-axis values were obtained by taking log2 from summed peptide peak areas of STC1 protein in different samples. Paired t test P=.0004, with significance accepted at P≤.05. (B) Relative expression of STC1 and calcium-sensing receptor (CASR) mRNA in human endometrial tissue throughout the menstrual cycle in women with and without endometriosis. (C) Expression of STC1 and CASR mRNA in human endometrial tissue throughout the menstrual cycle in women with endometriosis normalized to samples from women without endometriosis and expressed as fold change. ESE = early secretory phase endometrium; MSE = midsecretory phase endometrium; PE = proliferative phase endometrium. Nonparametric one-way analysis of variance Kruskal-Wallis test was used for statistical analysis, and significance was accepted at P≤.05. Fertility and Sterility 2016 106, 681-691.e1DOI: (10.1016/j.fertnstert.2016.05.023) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Immunohistochemical evaluation of STC1 protein expression in midsecretory human endometrium from women without and with endometriosis. LE = luminal epithelium; GE = glandular epithelium. *Statistically significant difference between LE and GE and between GE and stroma: P<.02. #Statistically significant difference in stromal staining between no endometriosis and endometriosis samples: P=.04. Magnification ×200. One-way Kruskal-Wallis test was used for statistical analysis, with significance accepted at P≤.05. (B) Immunohistochemical evaluation of CASR protein expression in mid-secretory human endometrium from women without and with endometriosis. No stromal expression of CASR was observed, and no difference in epithelial (luminal and glandular) staining was noted between specimens from women with versus without endometriosis. Magnification ×200. Abbreviations as in Figure 1. Fertility and Sterility 2016 106, 681-691.e1DOI: (10.1016/j.fertnstert.2016.05.023) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Expression of STC1 mRNA in decidualized human endometrial stromal fibroblasts (eSF) with (A) 0.5 mmol/L cyclic adenosine monophosphate (cAMP) for 96 hours, normalized to 96-hour control, or (B) 10 nmol/L E2/1 μmol/L P for 14 days, normalized to 14-day control. One-way Kruskal-Wallis test was used for statistical analysis, with significance accepted at P≤.05. Abbreviations as in Figure 1. Fertility and Sterility 2016 106, 681-691.e1DOI: (10.1016/j.fertnstert.2016.05.023) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions