D. R. Seifer, B. A. , B. D. Furman, M. S. , F. Guilak, Ph. D. , S. A

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Presentation transcript:

Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice D.R. Seifer, B.A., B.D. Furman, M.S., F. Guilak, Ph.D., S.A. Olson, M.D., S. Carroll Brooks, Ph.D., V. Byers Kraus, M.D., Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 12, Pages 1532-1538 (December 2008) DOI: 10.1016/j.joca.2008.04.013 Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Polyacrylate absorbance and recovery. (a, b) Mean volume of dH2O absorbed by a single PAB-L (n=30) over time; (c) mean volume of dH2O recovered from a single PAB (n=30). (d) Preswelling PAB-L increased the amount of protein recovered, n=3 per group (P=0.004, Independent Samples t test). Error bars represent a single SD. Osteoarthritis and Cartilage 2008 16, 1532-1538DOI: (10.1016/j.joca.2008.04.013) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Percent recovery comparison. (a, b) Comparisons between all six evaluated methodologies on percent recovery of total protein and KS, respectively. WPR was more efficient at protein recovery (a) and KS recovery (b) than CSAC with lyase (P=0.002, P=0.015, Independent Samples t test). Error bars represent a single SD. Osteoarthritis and Cartilage 2008 16, 1532-1538DOI: (10.1016/j.joca.2008.04.013) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Mean SF COMP levels in mouse knees. Total COMP (left panel) and COMP concentration (right panel) were highest in fractured left knees compared with non-fractured right knees in HFD fed mice. This difference in the fracture mice was statistically significant for total COMP (P=0.046). Osteoarthritis and Cartilage 2008 16, 1532-1538DOI: (10.1016/j.joca.2008.04.013) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Effects of cross-linkage on PAB properties. Varying levels of cross-linkage and their theoretically approximated effects on the absorption/oscillation curve of a PAB. Osteoarthritis and Cartilage 2008 16, 1532-1538DOI: (10.1016/j.joca.2008.04.013) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions