The chondrogenic repair response of undifferentiated mesenchymal cells in rat full- thickness articular cartilage defects Y. Anraku, M.D., H. Mizuta,

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The chondrogenic repair response of undifferentiated mesenchymal cells in rat full- thickness articular cartilage defects Y. Anraku, M.D., H. Mizuta, M.D., Ph.D., A. Sei, M.D., Ph.D., S. Kudo, M.D., Ph.D., E. Nakamura, M.D., Ph.D., K. Senba, M.D., Ph.D., K. Takagi, M.D., Ph.D., Y. Hiraki, Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 8, Pages 961-964 (August 2008) DOI: 10.1016/j.joca.2007.12.009 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 A custom-built twin-blade device for creating full-thickness defects in rat articular cartilage. (A) The device consists of two components: (1) the trunk (arrow), which has two slits to enable the twin-blades to pass through uniformly, and the contact point of the slit is curved (arrowhead) to fit along the femoral patellar groove. (2) The fixator (open arrow) which has two screws that anchor the tibia and contains a hexagonal bolt that attaches the two components. (B) This custom device reproducibly creates both defects on the rat articular surface of the femoral trochlea. Gross appearance of the defects at 2 days (C) and 4 weeks (D) post-injury. Bar, 5mm. Osteoarthritis and Cartilage 2008 16, 961-964DOI: (10.1016/j.joca.2007.12.009) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Transverse sections of the 0.7mm cartilage defects. The distal portion of the rat femur was cut in a transverse plane, and sections were stained with safranin-O at 2 days (A) 4 days (B) 7 days (C) 2 weeks (F) 4 weeks (G) and 24 weeks (J) after the creation of the defects. The specimen sections shown in (H) and (K) were also stained with anti-type II collagen monoclonal antibodies, shown in (G) and (J), respectively. The specimen sections shown in (I) and (L) were also stained with anti-aggrecan monoclonal antibodies in (H) and (K). In situ hybridization of type II collagen and aggrecan mRNAs in the 0.7mm defects at 7 days (D and E) post-injury. Bar, 300μm. Osteoarthritis and Cartilage 2008 16, 961-964DOI: (10.1016/j.joca.2007.12.009) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions