The CaptureSMB process principle

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Presentation transcript:

The CaptureSMB process principle Maximizing the efficiency of affinity capture steps Click to proceed

CaptureSMB process principle Loading of a batch column With CaptureSMB feed unused resin capacity elution feed fully loaded column presentation progress

Contichrom and CaptureSMB explained

Contichrom and CaptureSMB explained Feed Wasted resin capacity in batch chromatography Internal concentration t3 t2 Feed t1 column length

Contichrom and CaptureSMB explained Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil.

Contichrom and CaptureSMB explained Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil.

Contichrom and CaptureSMB explained Wash Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil.

Contichrom and CaptureSMB explained Elution Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. Product

Contichrom and CaptureSMB explained CIP & Re-equilibration Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil.

Contichrom and CaptureSMB explained Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil.

Contichrom and CaptureSMB explained Wash Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil.

Contichrom and CaptureSMB explained Feed Elution Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. Product

Contichrom and CaptureSMB explained Feed CIP & re-equil. Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. Start over

Overview CaptureSMB

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