A Novel Method for Preserving Human Lungs Using a Super-Cooling System

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A Novel Method for Preserving Human Lungs Using a Super-Cooling System Masayoshi Abe, MD, PhD, Shiro Jimi, PhD, Hiroshi Hama, Takeshi Shiraishi, MD, PhD, Akinori Iwasaki, MD, PhD, Nobuhumi Ono, PhD, Takayuki Shirakusa, MD, PhD, Takeshi Katsuragi, PhD The Annals of Thoracic Surgery Volume 82, Issue 3, Pages 1085-1088 (September 2006) DOI: 10.1016/j.athoracsur.2006.03.016 Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Histologic study to compare a novel and conventional preservation of isolated lungs. The lung tissue from each patient with lung cancer (ie, cases 1 to 3) was suspended in Euro-Collins solution and was kept at 4°C or at −5°C for 5 days. Thereafter the tissue was fixed and stained. Representative results of three cases is shown. (A–C) The tissue was stored at 4°C. (D–F) The tissue stored at −5°C were stained with hematoxylin-eosin (HE; left panel) and with anti-single-stranded DNA (ssDNA) antibody (right panel, brown-colored). The lung tissue stored at 4°C showed degenerative changes in (A) the alveoli, (B) the bronchi, and (C) the blood vessels. The bronchial epithelium (arrows) was severely affected (inserted square). In contrast, the tissues stored at −5°C, including (D) the alveoli, (E) the bronchi, and (F) the blood vessels were well preserved (inserted square). (A–C) Single-stranded DNA was frequently found in tissues stored at 4°C, but (D–F) was almost absent in tissues stored at −5°C. Original magnification ×200 except for insets (×400). The Annals of Thoracic Surgery 2006 82, 1085-1088DOI: (10.1016/j.athoracsur.2006.03.016) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Cysteinyl-leukotriene synthesis in chopped lung fragments caused by anaphylactic reaction. Lung tissue removed from two patients (cases 4 and 5) was suspended in Euro-Collins solution and kept at −5°C (hatched column) or 4°C (black column) for 3 days. Thereafter the lung fragments were cut into small pieces with scissors and then incubated with human immunoglobulin E at 22°C for 15 hours for passive sensitization. After washing with Tyrode’s buffer, the passively sensitized lung fragments were stimulated with or without anti-human immunoglobulin-E antibody (7.75 μg/nL) at 37°C for 30 minutes in the presence or absence of the patients’ serum (n = 4). After the termination of the reaction, cysLTs in the supernatants were assayed by purification with column chromatography and enzyme immunoassay. Data are reported as the mean ± standard error of the mean. The statistical analysis was performed using analysis of variance, and the Tukey-Kramer test was used for multiple comparisons (*p < 0.05). (αIgE ab = anti-immunoglobulin E-antibody.) The Annals of Thoracic Surgery 2006 82, 1085-1088DOI: (10.1016/j.athoracsur.2006.03.016) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions