Supplementary figure S2

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Supplementary figure S2 GAPDH Fibronectin N-Cadherin ZEB1 ZEB2 Slug 30 kDa Snail 29 kDa Twist 28 kDa β-actin 44 kDa HFF pBabe E6/E7 HFK 130 kDa 240 kDa 124 kDa 157 kDa 37 kDa B Slug Slug/DAPI Normal HPV +ve Tumour HPV -ve 100µm Inset Snail/Twist/DAPI Inset Normal HPV +ve HPV -ve Tumour 100µm Figure S2: (A) Immunofluorescence detection of Slug, Snail and Twist proteins in normal epithelium and tumour areas from HPV+ve and HPV-ve OPSCC. N=5 HPV(+ve) and 6 HPV(-ve) tumour sections. (B) Protein expressions of Slug, Snail, Twist, N-cadherin, Fibronectin, ZEB-1, ZEB-2, GAPDH and β-actin in human foreskin fibroblast (HFF) and human foreskin keratinocytes (HFK) expressing either empty vector (pBabe) or HPV16 E6/E7 genes.