Accuracy, Safety, and Tolerability of Tissue Collection by Cytosponge vs Endoscopy for Evaluation of Eosinophilic Esophagitis  David A. Katzka, Debra M. Geno, Anupama Ravi, Thomas C. Smyrk, Pierre Lao-Sirieix, Ahmed Miramedi, Irene Debiram, Maria O’Donovan, Hirohito Kita, Gail M. Kephart, Lori A. Kryzer, Michael Camilleri, Jeffrey A. Alexander, Rebecca C. Fitzgerald  Clinical Gastroenterology and Hepatology  Volume 13, Issue 1, Pages 77-83.e2 (January 2015) DOI: 10.1016/j.cgh.2014.06.026 Copyright © 2015 AGA Institute Terms and Conditions

Figure 1 Histology and EDN staining of biopsy and Cytosponge specimens from patient with positive findings. (A) Biopsy with mild esophageal eosinophilia (original magnification, ×20). (B) Cytosponge (original magnification, ×40) with esophageal eosinophilia. Note mild spongiosis present. (C) EDN staining biopsy showing both intracellular and extracellular staining, grade II (original magnification, ×20). (D) EDN staining Cytosponge demonstrating grade II staining extracellular and grade I intracellular (original magnification, ×40). Clinical Gastroenterology and Hepatology 2015 13, 77-83.e2DOI: (10.1016/j.cgh.2014.06.026) Copyright © 2015 AGA Institute Terms and Conditions

Figure 2 Histology and EDN staining of biopsy and Cytosponge specimens from patient with minimal or mild findings. (A) Biopsy (original magnification, ×40) with no eosinophils. (B) Cytosponge (original magnification, ×20) with scattered eosinophils also showing spongiosis and basal zone hyperplasia. (C) EDN staining biopsy (original magnification, ×40) with grade I intracellular and extracellular. (D) EDN staining Cytosponge (original magnification, ×20). Note grade II intranuclear staining present consistent with mild eosinophilia seen on histology. Clinical Gastroenterology and Hepatology 2015 13, 77-83.e2DOI: (10.1016/j.cgh.2014.06.026) Copyright © 2015 AGA Institute Terms and Conditions

Supplementary Figure 1 Scoring system for endoscopy and Cytosponge tolerance. Clinical Gastroenterology and Hepatology 2015 13, 77-83.e2DOI: (10.1016/j.cgh.2014.06.026) Copyright © 2015 AGA Institute Terms and Conditions