Effect of OPN on apoptosis of DCs

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Effect of OPN on apoptosis of DCs Effect of OPN on apoptosis of DCs. Immature DCs (imDCs) generated from monocytes incubated in the presence or absence of anti-OPN Ab (1, 3, or 5 μg/ml) were collected on day 5 and analyzed for apoptosis by annexin V and PI staining. Effect of OPN on apoptosis of DCs. Immature DCs (imDCs) generated from monocytes incubated in the presence or absence of anti-OPN Ab (1, 3, or 5 μg/ml) were collected on day 5 and analyzed for apoptosis by annexin V and PI staining. Anti-OPN Ab treatment increased apoptosis of immature DCs from 0.2% of immature control DCs to 8.4% of immature DCs treated with Ab (5 μg/ml). Immature DCs were collected and resuspended in new CM in the presence or absence of anti-OPN Ab and stimulated with LPS (1 μg/ml) for 48 h to obtain mature DCs (mDCs), and then mature DCs were collected and analyzed for apoptosis by annexin V and PI staining. Approximately 6.2% of induced mature DCs induced by LPS stimulation underwent apoptosis. Anti-OPN Ab treatment raised the rate of apoptosis to 50.1% in a dose-dependent manner. The results shown were obtained from a single experiment and are representative of three independent experiments that gave similar results. Kodai Kawamura et al. Clin. Diagn. Lab. Immunol. 2005; doi:10.1128/CDLI.12.1.206-212.2005