Fully differentiated human colon cancer HT-29 Glc−/+ cell subpopulation expressing the structural and functional characteristics of mature enterocytes.

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Fully differentiated human colon cancer HT-29 Glc−/+ cell subpopulation expressing the structural and functional characteristics of mature enterocytes of the small intestine. Fully differentiated human colon cancer HT-29 Glc−/+ cell subpopulation expressing the structural and functional characteristics of mature enterocytes of the small intestine. (A and B) Scanning electron microscopy examination shows the presence of a dense and well-organized brush border. (C) Transmission electron microscopy examination shows the well-organized microvilli. (D) Confocal laser scanning microscopy examination of SI immunofluorescence labeling shows the mosaic pattern of expression (x-y section). (E) Confocal laser scanning microscopy examination of SI immunofluorescence labeling shows the specific expression of hydrolase at the apical domain (x-z section). (F) Lack of SI immunofluorescence labeling in confluent undifferentiated parental HT-29 cells. Confluent cells at 21 days in culture are shown. Vanessa Liévin-Le Moal, and Alain L. Servin Microbiol. Mol. Biol. Rev. 2013; doi:10.1128/MMBR.00064-12