Development and clinical validation of a molecular diagnostic assay to detect CTX-M- type β-lactamases in Enterobacteriaceae  J.D.D. Pitout, N. Hamilton,

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Development and clinical validation of a molecular diagnostic assay to detect CTX-M- type β-lactamases in Enterobacteriaceae  J.D.D. Pitout, N. Hamilton, D.L. Church, P. Nordmann, L. Poirel  Clinical Microbiology and Infection  Volume 13, Issue 3, Pages 291-297 (March 2007) DOI: 10.1111/j.1469-0691.2006.01645.x Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 PCR assay for the detection of blaCTX-M genes. Lanes: L, 100-bp ladder; H, distilled water; N1, T1 (TEM-1); N2, T4 (TEM-10); N3, A1 (MOX-1); P1, CF2 (CTX-M-1); P2, Rio-4 (CTX-M-2); P3, Rio-3 (CTX-M-8); P4, CF1 (CTX-M-14); P5, ESBL530 (CTX-M-25); N4, S1 (SHV-2); N5, T1 (TEM-3); N6, A1 (MOX-1); and N7, A2 (KPC-1). rrs indicates the 16S rRNA internal control. Specific marker sizes in base pairs are shown on the left. More details of the individual strains are given in Table 1. Clinical Microbiology and Infection 2007 13, 291-297DOI: (10.1111/j.1469-0691.2006.01645.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

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