Intestinal phosphate absorption in a model of chronic renal failure J. Marks, L.J. Churchill, S.K. Srai, J. Biber, H. Murer, P. Jaeger, E.S. Debnam, R.J. Unwin  Kidney International  Volume 72, Issue 2, Pages 166-173 (July 2007) DOI: 10.1038/sj.ki.5002292 Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 1 Autoradiography (left section) and light field (right section) images of duodenum and jejunum from sham and 5/6-nephrectomy animals (bar=100 μm). Uptake was performed in vitro for 2 min using 100 μM32P phosphate. Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 2 In vivo uptake of phosphate by the duodenum and jejunum of sham-operated and 5/6-nephrectomized animals. Results are expressed as mean±s.e.m. of six experiments per region as a percentage of the initial counts transferred from 5 cm of small intestine into 1 ml of blood. **P<0.01, compared with sham jejunum and #P<0.05 compared with 5/6-nephrectomy jejunum using a one-way analysis of variance with post hoc comparisons performed using the Bonferroni multiple comparisons test. Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 3 Mucosal retention of phosphate in the duodenum and jejunum of sham-operated and 5/6-nephrectomized animals. Results are expressed as mean±s.e.m. of six experiments per region in nmol/g mucosa. *P<0.05, **P<0.01 compared with duodenum using a one-way analysis of variance with post hoc comparisons performed using the Bonferroni multiple comparisons test. Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 4 Real-time PCR quantification of NaPi-IIa mRNA in the kidney of sham and 5/6-nephrectomized rats. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIa is given as a ratio of NaPi-IIa mRNA to β-actin mRNA in arbitrary units (AU). ***P<0.001 using an unpaired t-test. Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 5 Real-time PCR quantification of NaPi-IIb mRNA in the duodenum and jejunum of sham and 5/6-nephrectomized rats. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIb is given as a ratio of NaPi-IIb mRNA to β-actin mRNA in arbitrary units (AU). Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 6 Real-time PCR quantification of NaPi-IIa mRNA in the kidney of 5/6-nephrectomized rats maintained on a normal or low-phosphate diet for 10 days. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIa is given as a ratio of NaPi-IIa mRNA to β-actin mRNA, in arbitrary units (AU). **P<0.01 using an unpaired t-test. Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 7 In vivo uptake of phosphate by the jejunum of 5/6-nephrectomized animals maintained on a normal or a low-phosphate diet for 10 days. Results are expressed as mean±s.e.m. of six experiments as a percentage of the initial counts transferred from 5 cm of small intestine into 1 ml of blood. Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 8 Real-time PCR quantification of NaPi-IIb mRNA in the duodenum and jejunum of 5/6-nephrectomized rats maintained on a normal or low-phosphate diet for 10 days. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIb is given as a ratio of NaPi-IIb mRNA to β-actin mRNA in arbitrary units (AU). Kidney International 2007 72, 166-173DOI: (10.1038/sj.ki.5002292) Copyright © 2007 International Society of Nephrology Terms and Conditions