Ex vivo IL-6 production. Ex vivo IL-6 production. Human whole blood was stimulated with the indicated doses of the E. coli O113 endotoxin that was used.

Slides:

Advertisements

Similar presentations

Characterization of inflammation and immune cell modulation induced by low- dose LPS administration to healthy volunteers M.R. Dillingh 1, E.P. van Poelgeest.

Advertisements

Serum Lactate Post-Antibiotic antibiotic † † ＊＊ Kumar et al. J Infect Dis 2006; 193:

Figure 4. Inhibition of LPS-induced IL-1β and TNFα, but not IL-6, after exercise. Depicted are LPS-induced IL-1β (upper panel), IL-6 (middle panel), and.

Extracellular ubiquitin inhibits the TNF-α response to endotoxin in peripheral blood mononuclear cells and regulates endotoxin hyporesponsiveness in critical.

Sensitivity, specificity, positive predictive value, and negative predictive value for the different cutoffs used. Sensitivity, specificity, positive predictive.

Bacteroidales LPS inhibits TLR stimulation.

Autoradiographs of cDNA gene array of regulatory genes from untreated control (column A) and quercetin-treated (column B) cells. Autoradiographs of cDNA.

Effect of quercetin on colony-forming abilities of prostate cancer cell lines with different aggressive potentials. Effect of quercetin on colony-forming.

Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays. Comparison of cytokine profiles induced.

Effect of OPN on apoptosis of DCs

Proinflammatory activities of Lactobacillus subcellular fractions.

Role of IL-27 in expression of PD-L1, LAG-3, and CTLA-4 depends on strength of α-CD3/28 signaling. Role of IL-27 in expression of PD-L1, LAG-3, and CTLA-4.

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

Hematological alterations after endotoxin injection.

Kinetics of CXC chemokine appearance after endotoxin injection.

Antibody endpoint titers induced by PfCelTOS vaccines containing various adjuvant formulations with emulsion doses of 0.5% to 2% (vol/vol) oil, as measured.

Profiles of anti-inflammatory cytokines after endotoxin injection.

Effect of route of administration (i.v. versus i.p.).

Effects of E. coli and S. aureus infection on levels of TNF-α and IL-1β in milk. Effects of E. coli and S. aureus infection on levels of TNF-α and IL-1β.

(A) CDK2 and p53 gene expression levels, as quantitated by real-time quantitative PCR with SYBR Green master mix (Stratagene, La Jolla, Calif.). (A) CDK2.

Figure 5 ADP-induced inflammatory responses require P2Y12 receptors in human microgliaIncreasing concentrations of ADP (5, 50, and 200 μM) increased tumor.

Treatment of dogs with celecoxib interferes with the effect of low-dose aspirin on ex vivo platelet aggregation. Treatment of dogs with celecoxib interferes.

ANCA-stimulated necroptosis-dependent NET generation induces NCGN

Platelet ATP secretion in response to agonist stimulation.

Volume 4, Issue 3, Pages (September 2008)

PCR amplification of the ORF encoding the cytosolic p36 protein of M

CD4+CLA+CD103+T cells from human blood and skin share a functional profile. CD4+CLA+CD103+T cells from human blood and skin share a functional profile.

IL-27 induces expression of multiple IR by CD8+ T cells.

Antibody endpoint titers induced by PfCelTOS vaccines containing various adjuvant formulations with emulsion doses of 0.02% to 2% (vol/vol) oil, as measured.

Memory-biased TCRs induce weaker TCR signals than effector-biased TCRs in vitro. Memory-biased TCRs induce weaker TCR signals than effector-biased TCRs.

Growth of B. dermatitidis yeast is inhibited by deferoxamine only at concentrations of 0.5 mM or greater. Growth of B. dermatitidis yeast is inhibited.

Overexpression of IL-27 upregulates expression of multiple IR by T cells in vivo. Overexpression of IL-27 upregulates expression of multiple IR by T cells.

Stress-induced release of inflammatory monocytes from the bone marrow into circulation was prevented by ADX. Male C57BL/6 mice were subjected to sham or.

Cytokine changes in LPS-induced TNFα and spontaneous IL-6 production in whole-blood cultures. Cytokine changes in LPS-induced TNFα and spontaneous IL-6.

IL-6 production with increasing doses of endotoxin.

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

The NTP increases tyrosine phosphorylation of PLC-γ1.

Schematic representation of the reactivities of IgM antibodies induced against Gly-Ala repeats of EBNA-1 and against glycine-rich motifs of HCMV antigens,

Comparison of OPN production by mature DCs and activated macrophages.

OPN mediates maturation from immature DCs to mature DCs

Reactivity of the anti-p36 MAbs against whole M

Effects of intramammary infection with E. coli or S

Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Representative cytokine and cytotoxicity responses to CMV peptides on day 0 and day 7 in one HLA A2+ donor. Representative cytokine and cytotoxicity responses.

Eight weeks after primary i. n

Effect of HL60 cell age (expressed in cell passage) on opsonophagocytic activity. Effect of HL60 cell age (expressed in cell passage) on opsonophagocytic.

IgM-specific immunoblot analysis of different HCMV recombinant antigens with a selected serum sample from an HCMV-seronegative patient with acute EBV-induced.

Microcolonies of S. pneumoniae serotype 6B on a Todd-Hewitt agar-yeast extract plate with an overlay containing TTC and antibiotic after 18 h of incubation.

Dot plot comparison of EBNA-1 with recombinant antigens 52/3 (aa 297 to 433 of pUL44) and UL57/3 (aa 545 to 601 of pUL57) of HCMV, containing glycine-rich.

Comparison of in vivo activity of 4D5scFvZZ and 4D5scFv.

Human basophils are unresponsive to contact-dependent or contact-independent inhibition by Tregs. Human basophils are unresponsive to contact-dependent.

Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.

Group-averaged IL-10 levels (in pg/ml) from the Luminex assay for control, CFS with FM, and CFS without FM groups. Group-averaged IL-10 levels (in pg/ml)

Effect of bevacizumab on the proliferation of A2780 cells.

Effects of intramammary infection with E. coli or S

Flow cytometric gating strategy used to identify CD69 surface expression on CD4 (left paired columns) and CD8 (right paired columns) T lymphocytes. Flow.

Long-lived antibody-secreting cells in the bone marrow, induced by PfCelTOS vaccines containing various adjuvant formulations with emulsion doses of 0.02%

Long-lived antibody-secreting cells in the bone marrow, induced by PfCelTOS vaccines containing various adjuvant formulations with emulsion doses of 0.5%

Distributions of levels of antibodies to periodontal pathogens and plasma vitamin C concentrations. Distributions of levels of antibodies to periodontal.

Antigen-specific cytokine-producing cells (IFN-γ and IL-5) detected by ELISPOT assay 3 weeks after the 3rd immunization. Antigen-specific cytokine-producing.

Targeted bile acid metabolomics of murine ileal and cecal contents.

Quantitation of apoptosis in M. aviumsubsp

Immunoblot of scFv antibody detected with an anti-FLAG antibody.

OPN production by immature DCs and mature DCs treated with PAMPs

Mean reciprocal IFA titer results for serum samples from experimentally vaccinated animals. Mean reciprocal IFA titer results for serum samples from experimentally.

Amino acid sequence of antigenic fragments 52/3 and UL57/3 of pUL44 and pUL57, respectively, and sequence comparison by dot plot analysis. Amino acid sequence.

The NTP inhibits the AFC response to SRBC

Indirect immunofluorescence reactions on primate smooth muscle tissue for EMA detection depicting the following: (A) prozone reactions at low dilution.

Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs

Presentation transcript:

Ex vivo IL-6 production. Ex vivo IL-6 production. Human whole blood was stimulated with the indicated doses of the E. coli O113 endotoxin that was used in the human studies (lot 6) or murine studies (lot 5). Plasma was harvested after 6 h of stimulation, and IL-6 concentrations (Conc.) were determined. At each dose of endotoxin, both lots induced statistically similar amounts of IL-6. Each value is the mean ± SEM (error bar) for the indicated number of donors. Shannon Copeland et al. Clin. Diagn. Lab. Immunol. 2005; doi:10.1128/CDLI.12.1.60-67.2005