The SOP can detect WNV infection in vitro and in vivo.

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The SOP can detect WNV infection in vitro and in vivo. The SOP can detect WNV infection in vitro and in vivo. (A and B) Infected WNV cells were spiked into uninfected cells (A) or uninfected tissues (B), libraries were prepared on RNA according to the SOP, and the libraries were examined by Illumina MiSeq. (C and D) Footpad (C) and brain tissue (D) from WNV-infected mice were analyzed at 5, 10, and 29 days postinfection for WNV-specific sequence reads by Illumina HiSeq. (A) Data representing the ability of the SOP to identify WNV-specific reads from limiting dilutions of WNV-infected Vero cells spiked into uninfected 293T cells. Mapped and unmapped reads from each sample are displayed. (B) The SOP identifies WNV-specific reads from limiting dilutions of WNV-infected Vero cells spiked into uninfected mouse tissues (spleen and brain). Mapped and unmapped reads from each sample are shown. (C) WNV was detected in the footpad RNA of mice prepared according to the SOP at the indicated times postinfection. (D) WNV-specific reads can be detected from brain tissue RNA of mice at the indicated times postinfection. For panels C and D, three mice per group were analyzed, and WNV-mapped and unmapped reads are shown. Lindsey A. Moser et al. mSystems 2016; doi:10.1128/mSystems.00039-15