Photo-crosslinked alginate hydrogels support enhanced matrix accumulation by nucleus pulposus cells in vivo A.I. Chou, S.O. Akintoye, S.B. Nicoll Osteoarthritis.

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General Information Osteoarthritis and Cartilage

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Photo-crosslinked alginate hydrogels support enhanced matrix accumulation by nucleus pulposus cells in vivo A.I. Chou, S.O. Akintoye, S.B. Nicoll Osteoarthritis and Cartilage Volume 17, Issue 10, Pages 1377-1384 (October 2009) DOI: 10.1016/j.joca.2009.04.012 Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Methacrylated alginate hydrogels after 4 weeks in vivo. Ionically (A) and photo-crosslinked (B) hydrogels. Arrows indicate location of the hydrogel. Osteoarthritis and Cartilage 2009 17, 1377-1384DOI: (10.1016/j.joca.2009.04.012) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Chondroitin sulfate proteoglycan immunohistochemistry of ionically and photo-crosslinked alginate hydrogels after 4 weeks of in vitro and in vivo culture. (A) Ionically crosslinked alginate hydrogel cultured in vitro, (B) photo-crosslinked alginate hydrogel cultured in vitro, and (C) photo-crosslinked alginate hydrogel in vivo. Scale bar=100mm. Osteoarthritis and Cartilage 2009 17, 1377-1384DOI: (10.1016/j.joca.2009.04.012) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Quantitative gene expression of types I (COL I) and II (COL II) collagen and aggrecan (AGG) normalized to GAPDH expression at 4 and 8 weeks post-implantation. * denotes a significant difference (P<0.05) between 4 and 8 weeks. Osteoarthritis and Cartilage 2009 17, 1377-1384DOI: (10.1016/j.joca.2009.04.012) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Total type II collagen (A) and sulfated GAG (B) accumulation normalized to the wet weight of the hydrogels at 4 and 8 weeks post-implantation. * denotes a significant difference between 4 and 8 weeks. Osteoarthritis and Cartilage 2009 17, 1377-1384DOI: (10.1016/j.joca.2009.04.012) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Immunohistochemistry of type II collagen (A, C) and chondroitin sulfate proteoglycan (B, D) ECM elaboration of photo-crosslinked alginate hydrogels at 4 (A, B) and 8 (C, D) weeks post-implantation. Scale bar=100μm. Osteoarthritis and Cartilage 2009 17, 1377-1384DOI: (10.1016/j.joca.2009.04.012) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Equilibrium Young's Modulus of photo-crosslinked alginate hydrogels cultured in vivo (A). Representative stereomicrographs of NP cell-encapsulated, photo-crosslinked alginate hydrogels pre-implantation (B), at 4 weeks post-implantation (C), and at 8 weeks post-implantation (D). Scale in mm. * denotes a significant difference (P<0.05) between cell-encapsulated and day 0 controls. Osteoarthritis and Cartilage 2009 17, 1377-1384DOI: (10.1016/j.joca.2009.04.012) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions