Effect of Ex Vivo Culture of CD34+ Bone Marrow Cells on Immune Reconstitution of XSCID Dogs Following Allogeneic Bone Marrow Transplantation Douglas.

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Effect of Ex Vivo Culture of CD34+ Bone Marrow Cells on Immune Reconstitution of XSCID Dogs Following Allogeneic Bone Marrow Transplantation Douglas R. Kennedy, Kyle McLellan, Peter F. Moore, Paula S. Henthorn, Peter J. Felsburg Biology of Blood and Marrow Transplantation Volume 15, Issue 6, Pages 662-670 (June 2009) DOI: 10.1016/j.bbmt.2009.03.014 Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 T cell reconstitution following DLA-matched or haploidentical bone marrow transplantation of XSCID dogs. Absolute number of peripheral CD45RA+ T cells in XSCID dogs following transplantation with ≥5 x 106 CD34+ bone marrow cells from normal matched (n=10) or haploidentical (n=6) donors. Biology of Blood and Marrow Transplantation 2009 15, 662-670DOI: (10.1016/j.bbmt.2009.03.014) Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Longitudinal analysis of T cell reconstitution following bone marrow transplantation of XSCID dogs with cultured bone marrow CD34+ cells. Proportion of peripheral T cells (A), and proportion (B) and absolute number (C) of peripheral T cells expressing a naïve (CD45RA+) phenotype in XSCID dogs following transplantation with CD34+ bone marrow cells following culture for 18 hours or 4.5 days in comparison with 8 dogs transplanted with freshly isolated CD34+ cells in Fig1, which that were incorporated into our breeding program. Biology of Blood and Marrow Transplantation 2009 15, 662-670DOI: (10.1016/j.bbmt.2009.03.014) Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 T cell function following bone marrow transplantation. Proliferative response of peripheral blood T cells following PHA stimulation in dogs R1789, X299, R1844, and R1847 at 4 months post BMT. The results of a normal dog analyzed at the same time are also presented. Percentages represent the proportion BrdU+ cells. Biology of Blood and Marrow Transplantation 2009 15, 662-670DOI: (10.1016/j.bbmt.2009.03.014) Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 4 Thymus output following bone marrow transplantation. TREC levels in XSCID dogs following transplantation with freshly isolated CD34+ cells or CD34+ cells cultured for 18hours or 4.5 days (A). Histologic appearance of the thymus in XSCID dogs one year following transplantation with freshly isolated bone marrow CD34+ cells or CD34+ cells cultured for 18hours or 4.5 days (B). Biology of Blood and Marrow Transplantation 2009 15, 662-670DOI: (10.1016/j.bbmt.2009.03.014) Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions