Dietary fatty acids differentially affect the development of injury-induced osteoarthritis with diet-induced obesity in mice C.-L. Wu, D. Jain, D. Little,

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Dietary fatty acids differentially affect the development of injury-induced osteoarthritis with diet-induced obesity in mice C.-L. Wu, D. Jain, D. Little, J.A. Anderson, J.L. Huebner, V.B. Kraus, R.M. Rodriguiz, W.C. Wetsel, F. Guilak Osteoarthritis and Cartilage Volume 21, Pages S224-S225 (April 2013) DOI: 10.1016/j.joca.2013.02.464 Copyright © 2013 Terms and Conditions

Figure 1 Representative microCT 3D reconstructions of mouse limbs at 28 weeks: (A) non-operated (right) hind limb from SFA mice (F = femur, T = tibia, P = patella; black arrows indicate partially calcified menisci) (B) Operated (left) joints of ω6 and SFA mice had increased heterotopic bone formation in the joint space (white arrows), as compared to the operated joints of Control and ω3 mice. Scale bar is 1 mm. Osteoarthritis and Cartilage 2013 21, S224-S225DOI: (10.1016/j.joca.2013.02.464) Copyright © 2013 Terms and Conditions

Figure 2 Assessment of OA severity. (A) Representative joint histology of the medial tibiofemoral contact region in left (DMM surgery) hind limbs (F = femur, M= meniscus, T = tibia). Severe cartilage cleft and loss were found in the joints of ω6 and SFA mice. Scale bar is 500 μm (B) Modified Mankin scores representative of OA severity. Data was analyzed by two-factor (diet and surgery) repeated measures ANOVA. Both factors and their interaction were significant (p < 0.01). Tukey's HSD post-hoc test on diet showed that the joints from Control and ω3 diet had significantly lower score compared to the joints from ω6 and SFA diet (p < 0.05). All operated limbs had significantly higher score than their corresponding control non-operated (right) joints (p < 0.01) and the operated joints of ω6 and SFA mice also had worse OA as compared to the operated joints of Control and ω3 mice (* p < 0.05). n ≥ 11 mice per diet group. Data shown as mean ± standard error. Osteoarthritis and Cartilage 2013 21, S224-S225DOI: (10.1016/j.joca.2013.02.464) Copyright © 2013 Terms and Conditions

Figure 3 Assessment of joint synovitis. (A) Representative joint histology of the medial femoral condyle of left (DMM surgery) hind limbs (F = femur, M= meniscus, T = tibia, S = synovium). Thickened synovium from ω6 and SFA diet with a high number of infiltrated cells was observed (indicated by black arrows). Scale bar is 500 μm. (B) Total Joint synovitis scores are representative of the degree of synovial inflammation. Data was analyzed by two-factor (diet and surgery) repeated measures ANOVA. Both factors but not their interaction were significant (p < 0.01). Tukey's HSD post-hoc test on diet showed that the joints from ω3 diet had significantly lower synovial inflammation compared to the joints from SFA and ω6 diet (* p < 0.05). n ≥ 11 mice per diet group. Data shown as mean ± standard error. Osteoarthritis and Cartilage 2013 21, S224-S225DOI: (10.1016/j.joca.2013.02.464) Copyright © 2013 Terms and Conditions