Autoantibodies to Collagen XVII Are Present in Parkinson’s Disease and Localize to Tyrosine-Hydroxylase Positive Neurons  Kelly A.N. Messingham, Samantha.

Slides:



Advertisements
Similar presentations
Methods Introduction Results Collagen XVII-specific autoantibodies in sera from patients with Parkinson's disease reacts with the neuronal, but not the.
Advertisements

2015 Alzheimer's disease facts and figures Alzheimer's & Dementia: The Journal of the Alzheimer's Association Volume 11, Issue 3, Pages (March.
Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters  Artem Vorobyev, Hideyuki Ujiie, Andreas Recke,
All-Cause and Cause-Specific Mortality in Patients with Psoriasis in Taiwan: A Nationwide Population-Based Study  Meng-Sui Lee, Yi-Chun Yeh, Yun-Ting.
Autoantibodies in Scurfy Mice and IPEX Patients Recognize Keratin 14
Journal of Allergy and Clinical Immunology
Suppression of Basophil FcεRI Activation by Serum from Active Chronic Idiopathic/Spontaneous Urticaria (CIU/CSU) Subjects  Patricia M. Sterba, Robert.
TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid  Yale Liu, Lingling Peng, Liang Li, Chengfei Liu, Xiao Hu, Shengxiang Xiao,
Super-Resolution Microscopy Reveals Altered Desmosomal Protein Organization in Tissue from Patients with Pemphigus Vulgaris  Sara N. Stahley, Maxine F.
The RD1-encoded antigen Rv3872 of Mycobacterium tuberculosis as a potential candidate for serodiagnosis of tuberculosis  P. Mukherjee, M. Dutta, P. Datta,
“Atypical p-ANCA” in IBD and hepatobiliary disorders react with a 50-kilodalton nuclear envelope protein of neutrophils and myeloid cell lines  Birgit.
Frequent Detection of Herpes Simplex Virus Antigen in Skin and Peripheral Blood CD34+ Mononuclear Cells from Patients with Graft-versus-Host Disease 
Fibroblast Activation Protein: Differential Expression and Serine Protease Activity in Reactive Stromal Fibroblasts of Melanocytic Skin Tumors  Margit.
Expression of Transglutaminase 5 in Normal and Pathologic Human Epidermis  Eleonora Candi, Sergio Oddi, Andrea Paradisi, Alessandro Terrinoni, Marco Ranalli,
The Antifibrotic Effect of α2AP Neutralization in Systemic Sclerosis Dermal Fibroblasts and Mouse Models of Systemic Sclerosis  Yosuke Kanno, En Shu,
Elevated Galectin-10 Expression of IL-22-Producing T Cells in Patients with Atopic Dermatitis  Seongmin Noh, Shan Jin, Chang Ook Park, Yun Sun Lee, Nara.
Epidermolysis Bullosa Acquisita Develops in Dominant Dystrophic Epidermolysis Bullosa  Ryota Hayashi, Ken Natsuga, Mika Watanabe, Hiroaki Iwata, Satoru.
Bullous Pemphigoid Sera that Contain Antibodies to BPAg2 also Contain Antibodies to LABD97 that Recognize Epitopes Distal to the NC16A Domain  Conleth.
Pathogenic Epitopes of Autoantibodies in Pemphigus Reside in the Amino-Terminal Adhesive Region of Desmogleins Which Are Unmasked by Proteolytic Processing.
Introduction to the Milestones in Autoimmune Bullous Diseases
C-Terminal Processing of Collagen XVII Induces Neoepitopes for Linear IgA Dermatosis Autoantibodies  Ellen Toyonaga, Wataru Nishie, Kentaro Izumi, Ken.
Epitopes in the Linker Subdomain Region of Envoplakin Recognized by Autoantibodies in Paraneoplastic Pemphigus Patients  Bingxin Zhang, Rui Zheng, Jing.
Loss of the Desmosomal Protein Perp Enhances the Phenotypic Effects of Pemphigus Vulgaris Autoantibodies  Bichchau Nguyen, Rachel L. Dusek, Veronica G.
Takashi Hashimoto, Norito Ishii, Daisuke Tsuruta 
Single Amino Acid Deletion in Kindlin-1 Results in Partial Protein Degradation Which Can Be Rescued by Chaperone Treatment  Kristin Maier, Yinghong He,
Marie-Thérèse Leccia  Journal of Investigative Dermatology 
Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing  Susanne Heiss, Rudolf Valenta 
Xiang Ding, Luis A. Diaz, Janet A. Fairley, George J. Giudice, Zhi Liu 
Nuclear Proteins and Apoptotic Bodies Are Found in the Lupus Band of Patients with Cutaneous Lupus Erythematosus  Karen Koopman, Tonnis R. Romeijn, Karina.
Volume 64, Issue 1, Pages 2-10 (July 2003)
Increased, but Functionally Impaired, CD14+ HLA-DR–/low Myeloid-Derived Suppressor Cells in Psoriasis: A Mechanism of Dysregulated T Cells  David C. Soler,
Anti-Desmoglein-1 Antibodies in Onchocerciasis, Leishmaniasis and Chagas Disease Suggest a Possible Etiological Link to Fogo Selvagem  Luis A. Diaz, Luis.
Autoantibodies in a Subgroup of Patients with Linear IgA Disease React with the NC16A Domain of BP1801  Detlef Zillikens, Karin Herzele, Matthias Georgi,
Function Blocking Autoantibodies Against Matrix Metalloproteinase-1 in Patients with Systemic Sclerosis  Shinichi Sato, Ikuko Hayakawa, Minoru Hasegawa,
Overexpression of CD109 in the Epidermis Differentially Regulates ALK1 Versus ALK5 Signaling and Modulates Extracellular Matrix Synthesis in the Skin 
Deimination of Human Hornerin Enhances its Processing by Calpain-1 and its Cross- Linking by Transglutaminases  Chiung-Yueh Hsu, Géraldine Gasc, Anne-Aurélie.
Autoantibodies to Bullous Pemphigoid Antigen 180 Induce Dermal–Epidermal Separation in Cryosections of Human Skin  Cassian Sitaru, Enno Schmidt, Steffen.
Detection of IgG Autoantibodies in the Sera of Patients with Bullous and Gestational Pemphigoid: ELISA Studies Utilizing a Baculovirus-Encoded Form of.
Prevalence of Antibodies Against Virus-Like Particles of Epidermodysplasia Verruciformis-Associated HPV8 in Patients at Risk of Skin Cancer  Sabine Stark,
Acrosin antibodies and infertility. I
Identification of a Potential Effector Function for IgE Autoantibodies in the Organ- Specific Autoimmune Disease Bullous Pemphigoid  Otobia G. Dimson,
Critical Role of Paxillin in Aging of Human Skin
Cloning of Hamster Type XVII Collagen cDNA, and Pathogenesis of Anti-Type XVII Collagen Antibody and Complement in Hamster Bullous Pemphigoid  Katsushi.
TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid  Yale Liu, Lingling Peng, Liang Li, Chengfei Liu, Xiao Hu, Shengxiang Xiao,
A Pathogenic Role for IgE in Autoimmunity: Bullous Pemphigoid IgE Reproduces the Early Phase of Lesion Development in Human Skin Grafted to nu/nu Mice 
Autoantibody Profile Differentiates between Inflammatory and Noninflammatory Bullous Pemphigoid  Kentaro Izumi, Wataru Nishie, Yosuke Mai, Mayumi Wada,
Scleroderma Fibroblasts Demonstrate Enhanced Activation of Akt (Protein Kinase B) In Situ  Jae-Bum Jun, Melanie Kuechle, Junki Min, Seung Cheol Shim,
Masaki Nagai, Minoru Hasegawa, Kazuhiko Takehara, Shinichi Sato 
Journal of Investigative Dermatology
Serum IgE Autoantibodies Target Keratinocytes in Patients with Atopic Dermatitis  Sabine Altrichter, Ernst Kriehuber, Julia Moser, Rudolf Valenta, Tamara.
Volume 13, Issue 3, Pages (March 2006)
IgG Autoantibodies from Bullous Pemphigoid (BP) Patients Bind Antigenic Sites on Both the Extracellular and the Intracellular Domains of the BP Antigen.
James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber 
The Prevalence and Clinical Significance of Anti-U1 RNA Antibodies in Patients with Systemic Sclerosis  Yoshihide Asano, Hironobu Ihn, Kenichi Yamane,
Production of Low Titers of Anti-Desmoglein 1 IgG Autoantibodies in Some Patients with Staphylococcal Scalded Skin Syndrome  Hidemi Anzai, John R. Stanley,
Alterations of Glucosylceramide-β-Glucosidase Levels in the Skin of Patients with Psoriasis Vulgaris  Francesca Alessandrini, Sabine Pfister, Elisabeth.
Autoantibody in Mucous Membrane Pemphigoid Binds to an Intracellular Epitope on Human β4 Integrin and Causes Basement Membrane Zone Separation in Oral.
IgG Autoantibodies from Bullous Pemphigoid Patients Recognize Multiple Antigenic Reactive Sites Located Predominantly Within the B and C Subdomains of.
Identification of HnRNP-A2/B1 as a Target Antigen of Anti-Endothelial Cell IgA Antibody in Behçet's Disease  Sung Bin Cho, Keun Jae Ahn, Do Hee Kim, Zhenlong.
Increased Levels of the Bullous Pemphigoid BP180 Autoantibody Are Associated with More Severe Dementia in Alzheimer’s Disease  Nina Kokkonen, Sanna-Kaisa.
Janet A. Fairley, Chang Ling Fu, George J. Giudice 
Detection of Laminin 5-Specific Auto-antibodies in Mucous Membrane and Bullous Pemphigoid Sera by ELISA  Vassiliki Bekou, Sybille Thoma-Uszynski, Olaf.
Severity and Phenotype of Bullous Pemphigoid Relate to Autoantibody Profile Against the NH2- and COOH-Terminal Regions of the BP180 Ectodomain  Silke.
Madison R. Mack, Gwen Wendelschafer-Crabb, Brian D. McAdams, Maria K
Volume 7, Issue 6, Pages (June 2014)
Flor Evangelista, David A. Dasher, Luis A. Diaz, Phillip S
Envoplakin and Periplakin, the Paraneoplastic Pemphigus Antigens, are also Recognized by Pemphigus Foliaceus Autoantibodies  Shideh Kazerounian, Mỹ G.
IgG anti-laminin-332 autoantibodies are present in a subset of patients with mucous membrane, but not bullous, pemphigoid  Zelmira Lazarova, MD, Valerie.
A user's perspective on GeoMxTM digital spatial profiling
Zhuxiang Nie, Takashi Hashimoto  Journal of Investigative Dermatology 
Presentation transcript:

Autoantibodies to Collagen XVII Are Present in Parkinson’s Disease and Localize to Tyrosine-Hydroxylase Positive Neurons  Kelly A.N. Messingham, Samantha Aust, Joseph Helfenberger, Krystal L. Parker, Susan Schultz, Julie McKillip, Nandakumar S. Narayanan, Janet A. Fairley  Journal of Investigative Dermatology  Volume 136, Issue 3, Pages 721-723 (March 2016) DOI: 10.1016/j.jid.2015.12.005 Copyright © 2015 The Authors Terms and Conditions

Figure 1 Serum reactivity to sec180 and NC16C regions of collagen XVII. Sera from 26 dementia and 24 Parkinson’s patients were screened for reactivity to recombinant sec180 or NC16C proteins by immunoblot. Reactivity was evaluated via densitometric analysis (NIH ImageJ) and values were normalized to the average density of negative control sera (assigned a value of 1, dotted line) and a 95% confidence interval (CI; dashed lines). (a) Sera from one dementia and seven Parkinson’s patients exhibited sec180 reactivity above the 95% CI and (b) sera from five dementia and two Parkinson’s patients exhibited reactivity to NC16C above the 95% CI. A single outlier (arrow) was identified using a Grubbs test (α = 0.01); omission of this outlier led to a significant (**P = 0.0027) difference in sec180 reactivity in dementia and Parkinson’s sera (Mann-Whitney test). No significant differences were observed in panel B. Journal of Investigative Dermatology 2016 136, 721-723DOI: (10.1016/j.jid.2015.12.005) Copyright © 2015 The Authors Terms and Conditions

Figure 2 Antibodies in Parkinson’s disease (PD) sera colocalize with dopaminergic neurons. Confocal images of cryosections of rat substantia nigra stained with anti-tyrosine hydroxylase (TH) antibodies (red) and PD sera (green). Using the colocalization finder tool in NIH Image J, areas of double staining in are pseudo-colored white; colocalization of IgG dopaminergic neurons (TH+) was observed with a blot-positive PD serum (a) but not a control PD serum (no binding of collagen XVII by blot) (b). To demonstrate the specificity of the co-localization, PD sera were pre-absorbed with gel-purified sec180 (-SEC180) or gel fragments devoid of protein (mock). The mock-absorption has no affect on co-localization of the signals (c) while preabsorption with sec180 results in a complete loss of co-localization (d). Images are representative of results obtained using sera from N = 5 individuals in each group. The scale bar = 100 μM. Journal of Investigative Dermatology 2016 136, 721-723DOI: (10.1016/j.jid.2015.12.005) Copyright © 2015 The Authors Terms and Conditions