Screening for acetaldehyde dehydrogenase 2 genotype in alcohol-induced asthma by using the ethanol patch test Hiroto Matsuse, MDa, Terufumi Shimoda,

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Presentation transcript:

Screening for acetaldehyde dehydrogenase 2 genotype in alcohol-induced asthma by using the ethanol patch test Hiroto Matsuse, MDa, Terufumi Shimoda, MDa, Chizu Fukushima, MDa, Kazuko Mitsuta, MDa, Tetsuya Kawano, MDa, Shin-ya Tomari, MDa, Sachiko Saeki, MDa, Yuki Kondoh, MDa, Ikuko Machida, MDa, Yasushi Obase, MDa, Sadahiro Asai, MDb, Shigeru Kohno, MDa Journal of Allergy and Clinical Immunology Volume 108, Issue 5, Pages 715-719 (November 2001) DOI: 10.1067/mai.2001.118791 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Representative PCR gel for ALDH2 genotypes. The ALDH2 genotype was determined by means of PCR with DNA isolated from peripheral blood (see “Methods” section). The 135 bp of PCR product is amplified with only normal primer (N) in type NN (normal homozygote), with both normal and mutant (M) primers in type NM (mutant heterozygote), and with only mutant primer in type MM (mutant homozygote). Journal of Allergy and Clinical Immunology 2001 108, 715-719DOI: (10.1067/mai.2001.118791) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Relationship between ethanol patch test and ALDH2 genotype. The distribution of ALDH2 genotype in asthmatic subjects with a positive or negative ethanol patch test result was determined with PCR. Results are shown as proportions (%) of types NN, NM, and MM. Journal of Allergy and Clinical Immunology 2001 108, 715-719DOI: (10.1067/mai.2001.118791) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Relationship between questionnaire and ALDH2 genotype. The distribution of ALDH2 genotype in asthmatic subjects with a positive or negative response to the part of the questionnaire addressing the past history of alcohol-induced asthma was determined by means of PCR. Results are shown as proportions (%) of types NN, NM, and MM. Journal of Allergy and Clinical Immunology 2001 108, 715-719DOI: (10.1067/mai.2001.118791) Copyright © 2001 Mosby, Inc. Terms and Conditions